Literature summary for 2.1.1.63 extracted from

Rasimas, J.J.; Kanugula, S.; Dalessio, P.M.; Ropson, I.J.; Fried, M.G.; Pegg, A.E.
Effects of zinc occupancy on human O6-alkylguanine-DNA alkyltransferase (2003), Biochemistry, 42, 980-990.

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Protein Variants

Protein Variants	Comment	Organism
C5A	smaller increase in stability on zinc addition than the wild-type hAGT	Homo sapiens
H29A	does not show the stability enehancement of wild-type hAGT with its intact zinc coordination sphere	Homo sapiens
H85A	does not show the stability enehancement of wild-type hAGT with its intact zinc coordination sphere	Homo sapiens

General Stability

General Stability	Organism
zinc provides conformational stability to hAGT that may influence its regulation	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zinc	in bacterial expression systems, recombinant hAGT is produced in increasingly larger quantities when growth media are supplemented with up to 0.1 mM ZnCl2. Metal-enriched hAGT samples have a 5fold increase in repair rate constant over conventionally purified protein samples and a 60fold increase over metal-stripped hAGT. Zinc confers a mechanistic enhancement to repair activity that does not result from an increase in substrate binding affinity. Zinc also provides conformational stability to hAGT that may influence its regulation	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Synonyms

Synonyms	Comment	Organism
hAGT	-	Homo sapiens

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Release 2023.1 (January 2023)