General Stability | Organism |
---|---|
enzyme is denatured by 8 M urea at pH 7.5 for 30 s or by 6 M guanidine hydrochloride at pH 7.5 for 16-24 h | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39356 | - |
x * 39356, inactive enzyme, electrospray mass spectrometry | Escherichia coli |
39377 | - |
x * 39377, active enzyme, electrospray mass spectrometry | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing 6-O-methylguanine + [protein]-L-cysteine | Escherichia coli | the enzyme functions in DNA repair by direct dealkylation of mutagenic 6-O-alkylguanine. The protein methylated at Cys69 becomes a transcriptional activator of the genes in the ada regulon, including its own | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
renaturation by dilution with buffer from 8 M urea or 6 M guanidine HCl instantly restores the secondary structure of the Ada protein to a state that is almost indistinguishable from the native state. Kinetics of renaturation is fast, at 0°C the restoration is quantitative in less than 30 s. The heat-coagulated protein can be restored to full activity by cycling it through treatment with 8 M urea or 6 M guanidine | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing 6-O-methylguanine + [protein]-L-cysteine | the enzyme functions in DNA repair by direct dealkylation of mutagenic 6-O-alkylguanine. The protein methylated at Cys69 becomes a transcriptional activator of the genes in the ada regulon, including its own | Escherichia coli | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39356, inactive enzyme, electrospray mass spectrometry | Escherichia coli |
? | x * 39377, active enzyme, electrospray mass spectrometry | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature-dependent inactivation of the enzyme is inhibited by DNA | Escherichia coli |
37 | - |
spontaneously loses about 30% of its secondary structure in less than 30 min concomitant with a complete loss of activity | Escherichia coli |
70 | - |
protein immediately coagulates | Escherichia coli |