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Literature summary for 2.1.1.63 extracted from

  • Roy, R.; Shiota, S.; Kennel, S.J.; Raha, R.; von Wronski, M.; Brent, T.P.; Mitra, S.
    A comparative study of the biochemical properties of human and mouse recombinant O6-methylguanine-DNA methyltransferases (1995), Carcinogenesis, 16, 405-411.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
DNA
-
Homo sapiens
DNA
-
Mus musculus
Na2SO4
-
Homo sapiens
Na2SO4
-
Mus musculus
Na3 citrate
-
Homo sapiens
Na3 citrate
-
Mus musculus
NaCl 0.2 M, 80% inhibition Homo sapiens
NaCl 0.2 M, wild-type and mutant enzyme, 55% inhibition Mus musculus
RNA
-
Homo sapiens
RNA
-
Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20900
-
x * 20900, SDS-PAGE Homo sapiens
22500
-
x * 22500, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA containing 6-O-methylguanine + [protein]-L-cysteine Mus musculus the enzyme repairs mutagenic and carcinogenic 6-O-alkylguanine in DNA DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine Homo sapiens the enzyme repairs mutagenic and carcinogenic 6-O-alkylguanine in DNA DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing 6-O-methylguanine + [protein]-L-cysteine enzyme reacts with the alkylated base in a synthetic DNA substrate poly(dC, dG, m6dG) Mus musculus DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine enzyme reacts with the alkylated base in a synthetic DNA substrate poly(dC, dG, m6dG) Homo sapiens DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine the enzyme repairs mutagenic and carcinogenic 6-O-alkylguanine in DNA Mus musculus DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine the enzyme repairs mutagenic and carcinogenic 6-O-alkylguanine in DNA Homo sapiens DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
?

Subunits

Subunits Comment Organism
? x * 20900, SDS-PAGE Homo sapiens
? x * 22500, SDS-PAGE Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
20 min, 55-65% loss of activity in absence of DNA, 25-35% loss of activity in presence of DNA, 0.2 mg/ml Mus musculus
50
-
20 min, 55-65% loss of activity in absence of DNA, 25-35% loss of activity in presence of DNA, 0.2 mg/ml Homo sapiens