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Literature summary for 2.1.1.6 extracted from

  • Mageroy, M.; Tieman, D.; Floystad, A.; Taylor, M.; Klee, H.
    A Solanum lycopersicum catechol-O-methyltransferase involved in synthesis of the flavor molecule guaiacol (2012), Plant J., 69, 1043-1051.
    View publication on PubMed
Search for more literature for 2.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00836
-
 catechol pH 7.5, 25°C Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + S-adenosyl-L-methionine
-
 Solanum lycopersicum guaiacol + S-adenosyl-L-homocysteine
-
 ?
additional information slight activity on orcinol, caffeic acid, and pyrogallol. No substrates: guaiacol, salicylic acid, benzoic acid, 2,5-dimethyl-4-methoxy-3(2H)-furanone Solanum lycopersicum ?
-
 ?
protocatechuic aldehyde + S-adenosyl-L-methionine 17% of the activity with catechol Solanum lycopersicum ? + S-adenosyl-L-homocysteine
-
 ?

Synonyms

Synonyms Comment Organism
CTOMT1
-
 Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.67
-
 catechol pH 7.5, 25°C Solanum lycopersicum

General Information

General Information Comment Organism
physiological function knockdown of isoform CTOMT1 results in significantly reduced fruit guaiacol emissions. CTOMT1 overexpression results in slightly increased fruit guaiacol emission. Upon supply with exogenously applied catechol, guaiacol production increases in both wild-type and transgenic fruit discs, although to a much greater extent in CTOMT1 overexpressing discs Solanum lycopersicum