Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant membrane-bound isoform, by arginine-affinity chromatography. Optimized conditions for isolation and purification consist in loading of 4 mg of total protein onto the column previously equilibrated at 20 °C and recovery of enzyme in a purified fraction using 500 mM NaCl, 10 mM DTT and 0.5% (v/v) Triton X-100 in 10 mM Tris buffer (pH 7) with a total bioactivity recovery of 24% and 4.95fold purification | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.092 | - |
pH 7.0, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epinephrine + S-adenosyl-L-methionine | - |
Homo sapiens | metanephrine + S-adenosyl-L-homocysteine | - |
? |