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Literature summary for 2.1.1.6 extracted from
- Crystal structures of rat catechol-O-methyltransferase complexed with coumarine-based inhibitor (2009), Biochem. Biophys. Res. Commun., 378, 494-497.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Codon Plus (DE3) cells | Rattus norvegicus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with 4-phenyl-7,8-dihydroxycoumarin, hanging drop vapor diffusion method, using 0.2 M (NH4)2SO4, 0.1 M Tris pH 7.5, 30% (w/v) PEG8000 | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-phenyl-7,8-dihydroxycoumarin | - |
Rattus norvegicus |  |
| additional information | COMT is a target for inhibitor development aiming at Parkinsons disease treatment and is submitted to extensive structure-based drug design | Rattus norvegicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mg2+ is a necessary cofactor for the methylation reaction, it is octahedrally coordinated in the active site by the side chains of D141, D169, N170, the two-hydroxyl groups of the catechol substrate, and a water molecule trans to N170 | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | P22734 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| GST 4B Sepharose resin column chromatography, Super Q-5PW column chromatography, and Superdex 75 gel filtration | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| S-COMT | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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