Cloned (Comment) | Organism |
---|---|
DNMT1, DNA and amino acid sequence determination and analysis, expression of wild-type and mutants in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of Dnmt1 mutants, two alleles of the endogenous Dnmt1 locus of R1 embryonic stem cells are sequentially modified using two similar targeting vectors to generate the DOX-OFF Dnmt1tet/tet cell line. The isolated 5' portion of the mouse Dnmt1 gene is used for targeted mutagenesis. Transient absence of DNMT1 expression leads to loss of imprinted methylation. In an embryonic stem cell line endogenous DNMT1s expression is extinguished by the addition of doxycycline, phenotypes, overview | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | Mus musculus | DNMT1 participates in epigenetic reprogramming through its ability to distinguish different categories of methylated sequences. Genomic imprinting is a mammalian epigenetic process that distinguishes maternal and paternal alleles to ensure parent-specific, monoallelic expression of imprinted genes. Preimplantation DNMT1-dependent maintenance mechanism specifically targets DMD sequences, e.g. of IAP, alpha-actin, Snurf/Snrpn, H19, Gnas, and Gtl2 DMD, overview | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
DNMT1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryonic stem cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | - |
Mus musculus | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? | |
DNA + S-adenosyl-L-methionine | DNMT1 participates in epigenetic reprogramming through its ability to distinguish different categories of methylated sequences. Genomic imprinting is a mammalian epigenetic process that distinguishes maternal and paternal alleles to ensure parent-specific, monoallelic expression of imprinted genes. Preimplantation DNMT1-dependent maintenance mechanism specifically targets DMD sequences, e.g. of IAP, alpha-actin, Snurf/Snrpn, H19, Gnas, and Gtl2 DMD, overview | Mus musculus | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNMT1 methyltransferase | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
physiological function | DNMT1 cytosine methyltransferase governs maintenance methylation in mammals, rearrangements of non-DMD, but not DMD methylation in preimplantation embryos | Mus musculus |