Any feedback?
Literature summary for 2.1.1.37 extracted from
- Allosteric activator domain of maintenance human DNA (cytosine-5) methyltransferase and its role in methylation spreading (2003), Biochemistry, 42, 5321-5332.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| methylated DNA | stimulated methylation spreading on unmethylated CpG sequences for full-length and the mutant lacking 121 N-terminal amino acids equally well. No stimulation of N-terminal deletion mutants lacking 501, 540, or 580 amino acids from the N-terminus | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Sf9 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTA1-501 | no stimulation by methylated DNA | Homo sapiens |
| DELTA1-540 | no stimulation by methylated DNA | Homo sapiens |
| DELTA1-580 | no stimulation by methylated DNA | Homo sapiens |
| H286A/R287A | allosteric activation by methylated DNA is reduced, 6.5 fold decrease in the ratio turnover number/Km-value compared to wild-type enzyme | Homo sapiens |
| K284A/K285A | allosteric activation by methylated DNA is reduced, 1.7fold decrease in the ratio turnover number/Km-value compared to wild-type enzyme | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| retinoblastoma gene product | a negative regulator of DNA methylation, binds to the allosteric site of hDNMT1 and inhibits methylation, it may regulate methylation spreading | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| 0.0005 | - |
poly-(dI-dC)/poly(dI-dC) | mutant enzyme K284A/K285A | Homo sapiens | |
| 0.00056 | - |
poly-(dI-dC)/poly(dI-dC) | wild-type enzyme | Homo sapiens | |
| 0.00082 | - |
poly-(dI-dC)/poly(dI-dC) | mutant enzyme H286A/R287A | Homo sapiens | |
| 0.0034 | - |
S-adenosyl-L-methionine | mutant enzyme H286A/R287A | Homo sapiens |  |
| 0.0072 | - |
S-adenosyl-L-methionine | wild-type enzyme | Homo sapiens | |
| 0.0092 | - |
S-adenosyl-L-methionine | mutant enzyme K284A/K285A | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
recombinant | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DNA | poly-(dI-dC)/poly(dI-dC), wild-type enzyme and N-terminal deletion mutants lacking 121, 501, 540, or 580 amino acids from the N-terminus methylate CG sequences, prefer hemimethylated to unmethylated DNA | Homo sapiens | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? | |
| S-adenosyl-L-methionine + poly-(dI-dC)/poly(dI-dC) | - |
Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hDNMT1 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| 0.013 | - |
S-adenosyl-L-methionine | mutant enzyme H286A/R287A | Homo sapiens | |
| 0.013 | - |
poly-(dI-dC)/poly(dI-dC) | mutant enzyme H286A/R287A | Homo sapiens | |
| 0.03 | - |
S-adenosyl-L-methionine | mutant enzyme K284A/K285A | Homo sapiens | |
| 0.03 | - |
poly-(dI-dC)/poly(dI-dC) | mutant enzyme K284A/K285A | Homo sapiens | |
| 0.058 | - |
S-adenosyl-L-methionine | wild-type enzyme | Homo sapiens | |
| 0.058 | - |
poly-(dI-dC)/poly(dI-dC) | wild-type enzyme | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
