Cloned (Comment) | Organism |
---|---|
DNA (cytosine C5) methyltransferase-1, fused to an amino-terminal hexahistidine tag, is expressed by infecting Spodoptera frugiperda cells for 46 h with a recombinant baculovirus carrying the DNA (cytosine-C5)methyltransferase-1 cDNA | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mus musculus | |
0.000973 | - |
poly(dIdC:dIdC) | - |
Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
190000 | - |
x * 190000, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | enzymatic DNA methylation of carbon 5 of cytosines is an epigenetic modification that plays a role in regulating gene expression, differentiation, and tumorigenesis. DNA (cytosine-C5)-methyltransferase-1 is the enzyme responsible for maintaining established methylation patterns during replication in mammalian cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mus musculus |
Storage Stability | Organism |
---|---|
-20°C, hexahistidine-tagged enzyme is stable for several months | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzymatic DNA methylation of carbon 5 of cytosines is an epigenetic modification that plays a role in regulating gene expression, differentiation, and tumorigenesis. DNA (cytosine-C5)-methyltransferase-1 is the enzyme responsible for maintaining established methylation patterns during replication in mammalian cells | Mus musculus | ? | - |
? | |
S-adenosyl-L-methionine + DNA | - |
Mus musculus | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? | |
S-adenosyl-L-methionine + DNA | the purified enzyme has at least a 30fold higher catalytic efficiency with hemimethylated double-stranded oligodeoxyribonucleotide substrates than unmethylated substrates and is most active with small oligodeoxyribonucleotide substrates | Mus musculus | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? | |
S-adenosyl-L-methionine + DNA | poly(dIdC:dIdC) is efficiently methylated | Mus musculus | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? | |
S-adenosyl-L-methionine + poly(dIdC:dIdC) | - |
Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 190000, SDS-PAGE | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mus musculus | |
0.0061 | - |
poly(dIdC:dIdC) | - |
Mus musculus |