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Literature summary for 2.1.1.37 extracted from

  • Pfeifer, G.P.; Grunwald, S.; Palitti, F.; Kaul, S.; Boehm, T.L.J.; Hirth, H.P.; Drahovsky, D.
    Purification and characterization of mammalian DNA methyltransferases by use of monoclonal antibodies (1985), J. Biol. Chem., 260, 13787-13793.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mus musculus
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0011
-
-
Mus musculus
0.00115
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, stable for at least 3 weeks Mus musculus
-80°C, stable for at least 3 weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA
-
Mus musculus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
?
S-adenosyl-L-methionine + DNA
-
Homo sapiens S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
?
S-adenosyl-L-methionine + DNA methylation of double-stranded Micrococcus luteus DNA. The enzyme transfers methyl groups to both double-stranded and single-stranded natural DNAs. The methyl-accepting activity of the DNA is correlated to their GC content, homologous DNAs are poor substrates. Very low rate of methylation with poly[(dA-dC)*(dG-dT)], highest methyl-accepting ability with poly[(dG-dC)*(dG-dC)] Mus musculus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
?
S-adenosyl-L-methionine + DNA methylation of double-stranded Micrococcus luteus DNA. The enzyme transfers methyl groups to both double-stranded and single-stranded natural DNAs. The methyl-accepting activity of the DNA is correlated to their GC content, homologous DNAs are poor substrates. Very low rate of methylation with poly[(dA-dC)*(dG-dT)], highest methyl-accepting ability with poly[(dG-dC)*(dG-dC)] Homo sapiens S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
rather unstable Mus musculus
37
-
rather unstable Homo sapiens