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Literature summary for 2.1.1.228 extracted from

  • Christian, T.; Evilia, C.; Hou, Y.M.
    Catalysis by the second class of tRNA(m1G37) methyl transferase requires a conserved proline (2006), Biochemistry, 45, 7463-7473.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
D223A kcat/Km for guanine37 in Methanocaldococcus jannaschii tRNACys is 1% of the wild-type value Methanocaldococcus jannaschii
G205A/G207A kcat/Km for guanine37 in Methanocaldococcus jannaschii tRNACys is 3% of the wild-type value Methanocaldococcus jannaschii
additional information proline at position 267 is a critical residue for catalysis, because substitution of this residue severely decreases kcat of the methylation reaction in steady-state kinetic analysis. However, substitution of P267 has milder effect on Km and little effect on Kd of either substrate. Because P267 has no functional side chain that can directly participate in the chemistry of methyl transfer, we suggest that its role in catalysis is to stabilize conformations of enzyme and substrates for proper alignment of reactive groups at the enzyme active site. Sequence analysis shows that P267 is embedded in a peptide motif that is conserved among the Trm5 family, but absent from the TrmD family, supporting the notion that the two families are descendants of unrelated protein structures Methanocaldococcus jannaschii
N225A kcat/Km for guanine37 in Methanocaldococcus jannaschii tRNACys is 5% of the wild-type value Methanocaldococcus jannaschii
N265A kcat/Km for guanine37 in Methanocaldococcus jannaschii tRNACys is 1% of the wild-type value Methanocaldococcus jannaschii
P226A kcat/Km for guanine37 in Methanocaldococcus jannaschii tRNACys is 6% of the wild-type value Methanocaldococcus jannaschii
P267A kcat/Km for guanine37 in Methanocaldococcus jannaschii tRNACys is 0.1% of the wild-type value Methanocaldococcus jannaschii
R144A kcat/Km for guanine37 in Methanocaldococcus jannaschii tRNACys is 6% of the wild-type value Methanocaldococcus jannaschii
Y176A kcat/Km for guanine37 in Methanocaldococcus jannaschii tRNACys is 5% of the wild-type value Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
S-adenosyl-L-methionine pH 8.0, 50°C, mutant enzyme P267A Methanocaldococcus jannaschii
0.0007
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, wild-type enzyme Methanocaldococcus jannaschii
0.001
-
S-adenosyl-L-methionine pH 8.0, 50°C, wild-type enzyme Methanocaldococcus jannaschii
0.0025
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme Y176A Methanocaldococcus jannaschii
0.003
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme D223A Methanocaldococcus jannaschii
0.003
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme P226A Methanocaldococcus jannaschii
0.0043
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme R144A Methanocaldococcus jannaschii
0.0046
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme G205A/G207A Methanocaldococcus jannaschii
0.006
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme P267A Methanocaldococcus jannaschii
0.0072
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme N225A Methanocaldococcus jannaschii
0.0083
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme N265A Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58293
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNACys
-
Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNACys
-
?

Synonyms

Synonyms Comment Organism
TRM5
-
Methanocaldococcus jannaschii
tRNA(m1G37) methyltransferase
-
Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0001
-
S-adenosyl-L-methionine pH 8.0, 50°C, mutant enzyme P267A Methanocaldococcus jannaschii
0.0001
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme P267A Methanocaldococcus jannaschii
0.0003
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme D223A Methanocaldococcus jannaschii
0.0013
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme N265A Methanocaldococcus jannaschii
0.0013
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme Y176A Methanocaldococcus jannaschii
0.0015
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme G205A/G207A Methanocaldococcus jannaschii
0.0018
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme P226A Methanocaldococcus jannaschii
0.003
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme R144A Methanocaldococcus jannaschii
0.0037
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme N225A Methanocaldococcus jannaschii
0.0083
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, wild-type enzyme Methanocaldococcus jannaschii
0.012
-
S-adenosyl-L-methionine pH 8.0, 50°C, wild-type enzyme Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Methanocaldococcus jannaschii

General Information

General Information Comment Organism
physiological function modification at guanine37 is important for maintaining the reading frame fidelity Methanocaldococcus jannaschii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.017
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme P267A Methanocaldococcus jannaschii
0.11
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme D223A Methanocaldococcus jannaschii
0.16
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme N265A Methanocaldococcus jannaschii
0.2
-
S-adenosyl-L-methionine pH 8.0, 50°C, mutant enzyme P267A Methanocaldococcus jannaschii
0.32
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme G205A/G207A Methanocaldococcus jannaschii
0.51
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme N225A Methanocaldococcus jannaschii
0.53
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme Y176A Methanocaldococcus jannaschii
0.61
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme P226A Methanocaldococcus jannaschii
0.775
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme R144A Methanocaldococcus jannaschii
11.9
-
guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, wild-type enzyme Methanocaldococcus jannaschii
12.2
-
S-adenosyl-L-methionine pH 8.0, 50°C, wild-type enzyme Methanocaldococcus jannaschii