Literature summary for 2.1.1.228 extracted from

Goto-Ito, S.; Ito, T.; Ishii, R.; Muto, Y.; Bessho, Y.; Yokoyama, S.
Crystal structure of archaeal tRNA(m(1)G37)methyltransferase aTrm5 (2008), Proteins, 72, 1274-1289.

Search for more literature for 2.1.1.228

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of Trm5 in complex with the methyl donor analogue sinefungin at 2.2 A resolution, vapor diffusion method at 20°C	Methanocaldococcus jannaschii

Inhibitors

Inhibitors	Comment	Organism	Structure
sinefungin	-	Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00028	-	guanine37 in Methanocaldococcus jannaschii tRNA(Cys)	pH 8.0, 50°C	Methanocaldococcus jannaschii

Organism

Organism	UniProt	Comment	Textmining
Methanocaldococcus jannaschii	Q58293	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNA(Cys)	-	Methanocaldococcus jannaschii	S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNA(Cys)	-	?

Synonyms

Synonyms	Comment	Organism
TRM5	-	Methanocaldococcus jannaschii
tRNA(m1G37)methyltransferase	-	Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	assay at	Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.01	-	guanine37 in Methanocaldococcus jannaschii tRNA(Cys)	pH 8.0, 50°C	Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Methanocaldococcus jannaschii

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
36.9	-	guanine37 in Methanocaldococcus jannaschii tRNA(Cys)	pH 8.0, 50°C	Methanocaldococcus jannaschii

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Release 2023.1 (January 2023)