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Literature summary for 2.1.1.228 extracted from
- Yeast mitochondrial initiator tRNA is methylated at guanosine 37 by the Trm5-encoded tRNA (guanine-N1-)-methyltransferase (2007), J. Biol. Chem., 282, 27744-27753.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as a fusion protein with maltose-binding protein | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
| mitochondrial matrix | - |
Saccharomyces cerevisiae | 5759 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + guanine37 in tRNA | Saccharomyces cerevisiae | Trm5p is responsible for m1G37 methylation of mitochondrial and cytoplasmic tRNAs | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P38793 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Trm5p is purified as a fusion protein with maltose-binding protein | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + guanine37 in tRNA | Trm5p is responsible for m1G37 methylation of mitochondrial and cytoplasmic tRNAs | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
| S-adenosyl-L-methionine + guanine37 in tRNA | methyltransferase activity with tRNA isolated from a DELTAtrm5 mutant strain, as well as with a synthetic mitochondrial initiator tRNA (tRNAMetf). N1-Methylguanine is determined by high pressure liquid chromatography analysis | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | the site of methylation is guanosine 37 in both mitochondrial tRNAMetf and tRNAPhe, determined by primer extension | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Trm5p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | efficiency or accuracy of mitochondrial protein synthesis is decreased in cells lacking m1G37 methylation of mitochondrial tRNAs | Saccharomyces cerevisiae |
| physiological function | the m1G37 tRNA modification plays an important role in reading frame maintenance in mitochondrial protein synthesis | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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