Cloned (Comment) | Organism |
---|---|
expression of His6-tagged Trm1 in Escherichia coli strain BL21 (DE3) | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AdoEnYn | a double-activated AdoMet analogue containing a pent-2-en-4-ynyl (EnYn) side chain instead of the methyl group. The double bond in beta-position to the sulfonium center counteracts unfavorable steric interactions within the SN2-like transition by conjugative stabilization and the terminal alkyne serves as reactive bio-orthogonal functionality. tRNAPhe methylation reaction to be inhibited by micromolar concentrations of AdoEnYn. EnYn-modified RNA structure determination by mass spectrometry | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNA | Pyrococcus furiosus | - |
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged Trm1 from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNA | - |
Pyrococcus furiosus | 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNA | - |
? | |
4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNAPhe | tRNAPhe from yeast | Pyrococcus furiosus | 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNAPhe | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Trm1 | - |
Pyrococcus furiosus |
tRNA:m22G26-methyltransferase | - |
Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Pyrococcus furiosus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.1 | - |
pH and temperature not specified in the publication | Pyrococcus furiosus | AdoEnYn |