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Literature summary for 2.1.1.215 extracted from

  • Motorin, Y.; Burhenne, J.; Teimer, R.; Koynov, K.; Willnow, S.; Weinhold, E.; Helm, M.
    Expanding the chemical scope of RNA:methyltransferases to site-specific alkynylation of RNA for click labeling (2011), Nucleic Acids Res., 39, 1943-1952.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged Trm1 in Escherichia coli strain BL21 (DE3) Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
AdoEnYn a double-activated AdoMet analogue containing a pent-2-en-4-ynyl (EnYn) side chain instead of the methyl group. The double bond in beta-position to the sulfonium center counteracts unfavorable steric interactions within the SN2-like transition by conjugative stabilization and the terminal alkyne serves as reactive bio-orthogonal functionality. tRNAPhe methylation reaction to be inhibited by micromolar concentrations of AdoEnYn. EnYn-modified RNA structure determination by mass spectrometry Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNA Pyrococcus furiosus
-
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNA
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged Trm1 from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNA
-
Pyrococcus furiosus 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNA
-
?
4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNAPhe tRNAPhe from yeast Pyrococcus furiosus 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNAPhe
-
?

Synonyms

Synonyms Comment Organism
Trm1
-
Pyrococcus furiosus
tRNA:m22G26-methyltransferase
-
Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Pyrococcus furiosus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.1
-
pH and temperature not specified in the publication Pyrococcus furiosus AdoEnYn