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Literature summary for 2.1.1.215 extracted from

  • Awai, T.; Kimura, S.; Tomikawa, C.; Ochi, A.; Ihsanawati, A.; Bessho, Y.; Yokoyama, S.; Ohno, S.; Nishikawa, K.; Yokogawa, T.; Suzuki, T.; Hori, H.
    Aquifex aeolicus tRNA (N2,N2-guanine)-dimethyltransferase (Trm1) catalyzes transfer of methyl groups not only to guanine 26 but also to guanine 27 in tRNA (2009), J. Biol. Chem., 284, 20467-20478.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00008
-
guanine27 in tRNATyr pH 7.5, 55°C, tRNA Tyr with wild-type sequence adenine26/guanine27 and mutation C43U Aquifex aeolicus
0.00009
-
guanine26 in mutant tRNATyr pH 7.5, 55°C, mutant tRNATyr in which the wilde-type adenine26/guanine27 sequence is substituted with guanine26/adenine27 Aquifex aeolicus
0.00009
-
guanine26 in mutant tRNATyr pH 7.5, 55°C, mutant tRNATyr in which the wilde-type adenine26/guanine27 sequence is substituted with guanine26/adenine27, additional point mutation C43U Aquifex aeolicus
0.00017
-
guanine27 in tRNATyr pH 7.5, 55°C, wild-type tRNATyr contains the sequence adenine26/guanine27 Aquifex aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNA Aquifex aeolicus
-
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNA
-
?
S-adenosyl-L-methionine + guanine26/guanine27 in tRNA Aquifex aeolicus
-
S-adenosyl-L-homocysteine + N2-methylguanine26/guanine27 in tRNA
-
?
S-adenosyl-L-methionine + N2-dimethylguanine26/guanine27 in tRNA Aquifex aeolicus
-
S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-methylguanine27 in tRNA
-
?
S-adenosyl-L-methionine + N2-dimethylguanine26/N2-methylguanine27 Aquifex aeolicus
-
S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27
-
?
S-adenosyl-L-methionine + N2-methylguanine26/guanine27 in tRNA Aquifex aeolicus
-
S-adenosyl-L-homocysteine + N2-dimethylguanine26/guanine27 in tRNA
-
?

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus O67010
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNA
-
Aquifex aeolicus 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNA
-
?
4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNACys m2G26 formation is faster than the m2G27 formation and disruption of the G27-C43 base pair accelerates velocity of the G27 modification. A fraction of native tRNACys has a N2-dimethylguanine26/N2-dimethylguanine27 modification. Initially the N2-methylguanine26 modification occurs, and then the second methyl transfer reaction generates N2-dimethylguanine26. The third methyl transfer reaction modifies guanine 27 to N2-methylguanine27 followed by a fourth methylation of N2-methylguanine27 to N2-dimethylguanine27 Aquifex aeolicus 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNACys
-
?
additional information recognition mechanisms of the Trm1 proteins: Aquifex aeolicus Trm1 recognizes the guanine26 and guanine27 bases from the T-arm. In contrast, archaeal and eukaryotic Trm1 proteins recognize the guanine26 base from the D-stem and variable region. The distance between the T-arm and guanine26 (or guanine27) is longer than the distance between the D-stem, variable region, and guanine26. These differences from binding site to the target guanine base(s) may decide the multisite or single site recognition of the Trm1 proteins Aquifex aeolicus ?
-
?
S-adenosyl-L-methionine + guanine26 in mutant tRNATyr mutant tRNATyr in which the wilde-type adenine26/guanine27 sequence is substituted with guanine26/adenine27 Aquifex aeolicus S-adenosyl-L-homocysteine + N2-methylguanine26 in mutant tRNATyr
-
?
S-adenosyl-L-methionine + guanine26/guanine27 in tRNA
-
Aquifex aeolicus S-adenosyl-L-homocysteine + N2-methylguanine26/guanine27 in tRNA
-
?
S-adenosyl-L-methionine + guanine26/guanine27 in tRNACys m2G26 formation is faster than the m2G27 formation and disruption of the G27-C43 base pair accelerates velocity of the G27 modification Aquifex aeolicus S-adenosyl-L-homocysteine + N2-methylguanine26/guanine27 in tRNACys
-
?
S-adenosyl-L-methionine + guanine27 in tRNATyr wild-type tRNATyr contains the sequence adenine26/guanine27 Aquifex aeolicus S-adenosyl-L-homocysteine + N2-methylguanine27 in tRNATyr
-
?
S-adenosyl-L-methionine + N2-dimethylguanine26/guanine27 in tRNA
-
Aquifex aeolicus S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-methylguanine27 in tRNA
-
?
S-adenosyl-L-methionine + N2-dimethylguanine26/guanine27 in tRNACys m2G26 formation is faster than the m2G27 formation and disruption of the G27-C43 base pair accelerates velocity of the G27 modification Aquifex aeolicus S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-methylguanine27 in tRNACys
-
?
S-adenosyl-L-methionine + N2-dimethylguanine26/N2-methylguanine27
-
Aquifex aeolicus S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27
-
?
S-adenosyl-L-methionine + N2-dimethylguanine26/N2-methylguanine27 in tRNACys m2G26 formation is faster than the m2G27 formation and disruption of the G27-C43 base pair accelerates velocity of the G27 modification Aquifex aeolicus S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNACys
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in mutant tRNATyr mutant tRNATyr in which the wilde-type adenine26/guanine27 sequence is substituted with guanine26/adenine27 Aquifex aeolicus S-adenosyl-L-homocysteine + N2-dimethylguanine26 in mutant tRNATyr
-
?
S-adenosyl-L-methionine + N2-methylguanine26/guanine27 in tRNA
-
Aquifex aeolicus S-adenosyl-L-homocysteine + N2-dimethylguanine26/guanine27 in tRNA
-
?
S-adenosyl-L-methionine + N2-methylguanine26/guanine27 in tRNACys m2G26 formation is faster than the m2G27 formation and disruption of the G27-C43 base pair accelerates velocity of the G27 modification Aquifex aeolicus S-adenosyl-L-homocysteine + N2-dimethylguanine26/guanine27 in tRNACys
-
?
S-adenosyl-L-methionine + N2-methylguanine27 in tRNATyr wild-type tRNATyr contains the sequence adenine26/guanine27 Aquifex aeolicus S-adenosyl-L-homocysteine + N2-dimethylguanine27 in tRNATyr
-
?

Synonyms

Synonyms Comment Organism
Trm1
-
Aquifex aeolicus
tRNA (N2,N2-guanine)-dimethyltransferase
-
Aquifex aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
assay at Aquifex aeolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Aquifex aeolicus