Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00008 | - |
guanine27 in tRNATyr | pH 7.5, 55°C, tRNA Tyr with wild-type sequence adenine26/guanine27 and mutation C43U | Aquifex aeolicus | |
0.00009 | - |
guanine26 in mutant tRNATyr | pH 7.5, 55°C, mutant tRNATyr in which the wilde-type adenine26/guanine27 sequence is substituted with guanine26/adenine27 | Aquifex aeolicus | |
0.00009 | - |
guanine26 in mutant tRNATyr | pH 7.5, 55°C, mutant tRNATyr in which the wilde-type adenine26/guanine27 sequence is substituted with guanine26/adenine27, additional point mutation C43U | Aquifex aeolicus | |
0.00017 | - |
guanine27 in tRNATyr | pH 7.5, 55°C, wild-type tRNATyr contains the sequence adenine26/guanine27 | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNA | Aquifex aeolicus | - |
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine26/guanine27 in tRNA | Aquifex aeolicus | - |
S-adenosyl-L-homocysteine + N2-methylguanine26/guanine27 in tRNA | - |
? | |
S-adenosyl-L-methionine + N2-dimethylguanine26/guanine27 in tRNA | Aquifex aeolicus | - |
S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-methylguanine27 in tRNA | - |
? | |
S-adenosyl-L-methionine + N2-dimethylguanine26/N2-methylguanine27 | Aquifex aeolicus | - |
S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 | - |
? | |
S-adenosyl-L-methionine + N2-methylguanine26/guanine27 in tRNA | Aquifex aeolicus | - |
S-adenosyl-L-homocysteine + N2-dimethylguanine26/guanine27 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O67010 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNA | - |
Aquifex aeolicus | 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNA | - |
? | |
4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNACys | m2G26 formation is faster than the m2G27 formation and disruption of the G27-C43 base pair accelerates velocity of the G27 modification. A fraction of native tRNACys has a N2-dimethylguanine26/N2-dimethylguanine27 modification. Initially the N2-methylguanine26 modification occurs, and then the second methyl transfer reaction generates N2-dimethylguanine26. The third methyl transfer reaction modifies guanine 27 to N2-methylguanine27 followed by a fourth methylation of N2-methylguanine27 to N2-dimethylguanine27 | Aquifex aeolicus | 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNACys | - |
? | |
additional information | recognition mechanisms of the Trm1 proteins: Aquifex aeolicus Trm1 recognizes the guanine26 and guanine27 bases from the T-arm. In contrast, archaeal and eukaryotic Trm1 proteins recognize the guanine26 base from the D-stem and variable region. The distance between the T-arm and guanine26 (or guanine27) is longer than the distance between the D-stem, variable region, and guanine26. These differences from binding site to the target guanine base(s) may decide the multisite or single site recognition of the Trm1 proteins | Aquifex aeolicus | ? | - |
? | |
S-adenosyl-L-methionine + guanine26 in mutant tRNATyr | mutant tRNATyr in which the wilde-type adenine26/guanine27 sequence is substituted with guanine26/adenine27 | Aquifex aeolicus | S-adenosyl-L-homocysteine + N2-methylguanine26 in mutant tRNATyr | - |
? | |
S-adenosyl-L-methionine + guanine26/guanine27 in tRNA | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N2-methylguanine26/guanine27 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine26/guanine27 in tRNACys | m2G26 formation is faster than the m2G27 formation and disruption of the G27-C43 base pair accelerates velocity of the G27 modification | Aquifex aeolicus | S-adenosyl-L-homocysteine + N2-methylguanine26/guanine27 in tRNACys | - |
? | |
S-adenosyl-L-methionine + guanine27 in tRNATyr | wild-type tRNATyr contains the sequence adenine26/guanine27 | Aquifex aeolicus | S-adenosyl-L-homocysteine + N2-methylguanine27 in tRNATyr | - |
? | |
S-adenosyl-L-methionine + N2-dimethylguanine26/guanine27 in tRNA | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-methylguanine27 in tRNA | - |
? | |
S-adenosyl-L-methionine + N2-dimethylguanine26/guanine27 in tRNACys | m2G26 formation is faster than the m2G27 formation and disruption of the G27-C43 base pair accelerates velocity of the G27 modification | Aquifex aeolicus | S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-methylguanine27 in tRNACys | - |
? | |
S-adenosyl-L-methionine + N2-dimethylguanine26/N2-methylguanine27 | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 | - |
? | |
S-adenosyl-L-methionine + N2-dimethylguanine26/N2-methylguanine27 in tRNACys | m2G26 formation is faster than the m2G27 formation and disruption of the G27-C43 base pair accelerates velocity of the G27 modification | Aquifex aeolicus | S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNACys | - |
? | |
S-adenosyl-L-methionine + N2-methylguanine26 in mutant tRNATyr | mutant tRNATyr in which the wilde-type adenine26/guanine27 sequence is substituted with guanine26/adenine27 | Aquifex aeolicus | S-adenosyl-L-homocysteine + N2-dimethylguanine26 in mutant tRNATyr | - |
? | |
S-adenosyl-L-methionine + N2-methylguanine26/guanine27 in tRNA | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N2-dimethylguanine26/guanine27 in tRNA | - |
? | |
S-adenosyl-L-methionine + N2-methylguanine26/guanine27 in tRNACys | m2G26 formation is faster than the m2G27 formation and disruption of the G27-C43 base pair accelerates velocity of the G27 modification | Aquifex aeolicus | S-adenosyl-L-homocysteine + N2-dimethylguanine26/guanine27 in tRNACys | - |
? | |
S-adenosyl-L-methionine + N2-methylguanine27 in tRNATyr | wild-type tRNATyr contains the sequence adenine26/guanine27 | Aquifex aeolicus | S-adenosyl-L-homocysteine + N2-dimethylguanine27 in tRNATyr | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Trm1 | - |
Aquifex aeolicus |
tRNA (N2,N2-guanine)-dimethyltransferase | - |
Aquifex aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Aquifex aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Aquifex aeolicus |