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Literature summary for 2.1.1.2 extracted from

  • Braissant, O.; Henry, H.; Villard, A.; Speer, O.; Wallimann, T.; Bachmann, C.
    Creatine synthesis and transport during rat embryogenesis: Spatiotemporal expression of AGAT, GAMT and CT1 (2005), BMC Dev. Biol., 5, 9.
    View publication on PubMedView publication on EuropePMC
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Organism

Organism UniProt Comment Textmining
Rattus norvegicus P10868 Sprague-Dawley, 12.5, 15.5 and 18.5 days of gestation
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Source Tissue

Source Tissue Comment Organism Textmining
brain in embryos at 15.5 days of gestation guanidinoacetate N-methyltransferase is detectable in pons and striatum. In embryos at 18.5 days of gestation guanidinoacetate N-methyltransferase is detectable in neocortex, hippocampus, striatum, pallidum and spinal cord. Non-radioactive in situ hybridization (58°C for 40 h in 5 x SSC, 50% formamide and 40 microg/ml salmon sperm DNA) with digoxigenin-labeled antisense and sense riboprobes (400 ng/ml) for rat guanidinoacetate N-methyltransferase. guanidinoacetate N-methyltransferase proteins are detected with rabbit polyclonal antibodies Rattus norvegicus
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hepatic primordium in embryos at 12.5 days of gestation guanidinoacetate N-methyltransferase is detectable in the hepatic primordium only, with all other tissues being negative. Non-radioactive in situ hybridization (58°C for 40 h in 5 x SSC, 50% formamide and 40 microg/ml salmon sperm DNA) with digoxigenin-labeled antisense and sense riboprobes (400 ng/ml) for rat guanidinoacetate N-methyltransferase. guanidinoacetate N-methyltransferase proteins are detected with rabbit polyclonal antibodies Rattus norvegicus
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intestinal epithelium in embryos at 18.5 days of gestation guanidinoacetate N-methyltransferase is detectable. Non-radioactive in situ hybridization (58°C for 40 h in 5 x SSC, 50% formamide and 40 microg/ml salmon sperm DNA) with digoxigenin-labeled antisense and sense riboprobes (400 ng/ml) for rat guanidinoacetate N-methyltransferase. guanidinoacetate N-methyltransferase proteins are detected with rabbit polyclonal antibodies Rattus norvegicus
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liver in embryos at 15.5 and 18.5 days of gestation guanidinoacetate N-methyltransferase is detectable in liver. Non-radioactive in situ hybridization (58°C for 40 h in 5 x SSC, 50% formamide and 40 microg/ml salmon sperm DNA) with digoxigenin-labeled antisense and sense riboprobes (400 ng/ml) for rat guanidinoacetate N-methyltransferase. guanidinoacetate N-methyltransferase proteins are detected with rabbit polyclonal antibodies Rattus norvegicus
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additional information guanidinoacetate N-methyltransferase can not be detected in kidney of embryos of 12.5, 15.5 and 18.5 days of gestation. Non-radioactive in situ hybridization (58°C for 40 h in 5 x SSC, 50% formamide and 40 microg/ml salmon sperm DNA) with digoxigenin-labeled antisense and sense riboprobes (400 ng/ml) for rat guanidinoacetate N-methyltransferase. guanidinoacetate N-methyltransferase proteins are detected with rabbit polyclonal antibodies Rattus norvegicus
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pancreas in embryos at 15.5 and 18.5 days of gestation guanidinoacetate N-methyltransferase is detectable in pancreas. Non-radioactive in situ hybridization (58°C for 40 h in 5 x SSC, 50% formamide and 40 microg/ml salmon sperm DNA) with digoxigenin-labeled antisense and sense riboprobes (400 ng/ml) for rat guanidinoacetate N-methyltransferase. guanidinoacetate N-methyltransferase proteins are detected with rabbit polyclonal antibodies Rattus norvegicus
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skeletal muscle in embryos at 18.5 days of gestation guanidinoacetate N-methyltransferase is detectable. Non-radioactive in situ hybridization (58°C for 40 h in 5 x SSC, 50% formamide and 40 microg/ml salmon sperm DNA) with digoxigenin-labeled antisense and sense riboprobes (400 ng/ml) for rat guanidinoacetate N-methyltransferase. guanidinoacetate N-methyltransferase proteins are detected with rabbit polyclonal antibodies Rattus norvegicus
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Synonyms

Synonyms Comment Organism
GAMT
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 Rattus norvegicus