Literature summary for 2.1.1.2 extracted from

Hamahata, A.; Takata, Y.; Gomi, T.; Fujioka, M.
Probing the S-adenosylmethionine-binding site of rat guanidinoacetate methyltransferase: effect of site-directed mutagenesis of residues that are conserved across mammalian non-nucleic acid methyltransferases (1996), Biochem. J., 317, 141-145.

No PubMed abstract available

Search for more literature for 2.1.1.2

Protein Variants

Protein Variants	Comment	Organism
D92N	lower activity than wild type enzyme	Rattus norvegicus
E89Q	lower activity than wild type enzyme	Rattus norvegicus
G67A	inactive enzyme	Rattus norvegicus
G69A	inactive enzyme	Rattus norvegicus
W143F	lower activity than wild type enzyme	Rattus norvegicus
W143L	lower activity than wild type enzyme	Rattus norvegicus
Y133V	lower activity than wild type enzyme	Rattus norvegicus
Y136F	higher turnover than wild type enzyme	Rattus norvegicus
Y136V	lower activity than wild type enzyme	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	all mutant enzymes listed with Km	Rattus norvegicus
0.005	-	S-adenosyl-L-methionine	wild type enzyme	Rattus norvegicus
0.011	-	Guanidinoacetate	wild type enzyme	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + guanidinoacetate	Rattus norvegicus	-	S-adenosyl-L-homocysteine + creatine	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanidinoacetate	-	Rattus norvegicus	S-adenosyl-L-homocysteine + creatine	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	all mutant enzymes listed with turnover	Rattus norvegicus
0.0788	-	Guanidinoacetate	wild type enzyme	Rattus norvegicus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)