Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | reducing agent required for activity | Pseudomonas sp. | |
ascorbate | can replace 2-mercaptoethanol, best reducing agent for reaction assay | Pseudomonas sp. | |
homocysteine | can replace 2-mercaptoethanol | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | inhibitory in excess | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
tetrahydrofolate | - |
Pseudomonas sp. | |
5.8 | - |
Trimethylsulfonium | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
sucrose density gradient centrifugation | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
atypical pseudomonad with two lateral, subpolar flagellae | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.007 | - |
purified enzyme | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, 80% activity retained after 4 months | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | S-adenosylmethionine and dimethyl-beta-propiothetin are not accepted as methyl donors | Pseudomonas sp. | ? | - |
? | |
trimethylsulfonium + tetrahydrofolate | - |
Pseudomonas sp. | dimethylsulfide + 5-methyltetrahydrofolate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
broad optimum | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrofolate | - |
Pseudomonas sp. |