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Literature summary for 1.97.1.2 extracted from
- Cloning, sequencing and heterologous expression of pyrogallol-phloroglucinol transhydroxylase from Pelobacter acidigallici (1999), Eur. J. Biochem., 265, 896-901.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain BL21 and M15 | Pelobacter acidigallici |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | iron-sulfur cluster | Pelobacter acidigallici |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31221 | - |
alpha,beta, 1 * 99260 + 1 * 31221, calculation from amino acid sequence | Pelobacter acidigallici |
| 99260 | - |
alpha,beta, 1 * 99260 + 1 * 31221, calculation from amino acid sequence | Pelobacter acidigallici |
| 130500 | - |
excluding cofactors, calculation from amino acid sequence | Pelobacter acidigallici |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2,3,5-tetrahydroxybenzene + pyrogallol | Pelobacter acidigallici | key step in the anaerobic degradation of gallic acid and releated polyphenolic compounds | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pelobacter acidigallici | - |
ATCC 49970 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2,3,5-tetrahydroxybenzene + pyrogallol | pyrogallol i.e. 1,2,3-tri-hydroxybenzene | Pelobacter acidigallici | phloroglucinol + 1,2,3,5-tetrahydroxybenzene | phloroglucinol i.e. 1,3,5-tri-hydroxybenzene | ? | |
| 1,2,3,5-tetrahydroxybenzene + pyrogallol | key step in the anaerobic degradation of gallic acid and releated polyphenolic compounds | Pelobacter acidigallici | ? | - |
? | |
| additional information | water is not the source of the added hydroxyl groups | Pelobacter acidigallici | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | alpha,beta, 1 * 99260 + 1 * 31221, calculation from amino acid sequence | Pelobacter acidigallici |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| molybdenum cofactor | - |
Pelobacter acidigallici | |
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