Cloned (Comment) | Organism |
---|---|
gene cysH, expression in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
General Stability | Organism |
---|---|
activity is lost upon exposure to air or upon dilution | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
3'-phosphoadenylyl-sulfate | recombinant enzyme, pH 8.0, with thioredoxin 1 | Bacillus subtilis | |
0.0107 | - |
3'-phosphoadenylyl-sulfate | recombinant enzyme, pH 8.0, with glutaredoxin 1 | Bacillus subtilis | |
0.0225 | - |
glutaredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate | Bacillus subtilis | |
0.0287 | - |
3'-phosphoadenylyl sulfate | recombinant enzyme, pH 8.0, with thioredoxin 1 | Bacillus subtilis | |
0.0543 | - |
thioredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate | Bacillus subtilis | |
0.0606 | - |
glutaredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate | Bacillus subtilis | |
0.0675 | - |
thioredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate | Bacillus subtilis | |
0.1054 | - |
3'-phosphoadenylyl sulfate | recombinant enzyme, pH 8.0, with glutaredoxin 1 | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the enzyme contains a [4Fe-4S] cluster per subunit, absolutely required for activity, constitution is altered in presence of oxygen, activity is restored after decomposition and reassembly | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
2 * 27000, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-adenylyl-sulfate + thioredoxin | Bacillus subtilis | - |
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? | |
3'-phosphoadenylyl-sulfate + glutaredoxin | Bacillus subtilis | - |
adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite | - |
? | |
3'-phosphoadenylyl-sulfate + thioredoxin | Bacillus subtilis | - |
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene cysH, bispecific enzyme | - |
Oxidation Stability | Organism |
---|---|
activity is lost upon exposure to air | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-adenylyl-sulfate + thioredoxin | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? | |
3'-phosphoadenylyl sulfate + glutaredoxin 1 | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + sulfite + glutaredoxin 1 disulfide | - |
r | |
3'-phosphoadenylyl sulfate + thioredoxin 1 | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + sulfite + thioredoxin 1 disulfide | - |
r | |
3'-phosphoadenylyl-sulfate + glutaredoxin | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite | - |
? | |
3'-phosphoadenylyl-sulfate + thioredoxin | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 27000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
4Fe-4S adenylyl sulfate/phosphoadenylyl sulfate reductase | - |
Bacillus subtilis |
CysH1 | - |
Bacillus subtilis |
SNR | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.146 | - |
glutaredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate | Bacillus subtilis | |
0.149 | - |
3'-phosphoadenylyl sulfate | recombinant enzyme, pH 8.0, with thioredoxin 1 | Bacillus subtilis | |
0.172 | - |
3'-phosphoadenylyl-sulfate | recombinant enzyme, pH 8.0, with thioredoxin 1 | Bacillus subtilis | |
0.174 | - |
thioredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate | Bacillus subtilis | |
0.219 | - |
thioredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate | Bacillus subtilis | |
0.335 | - |
3'-phosphoadenylyl sulfate | recombinant enzyme, pH 8.0, with glutaredoxin 1 | Bacillus subtilis | |
0.382 | - |
3'-phosphoadenylyl-sulfate | recombinant enzyme, pH 8.0, with glutaredoxin 1 | Bacillus subtilis | |
0.421 | - |
glutaredoxin 1 | recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutaredoxin | glutaredoxin 1 variant | Bacillus subtilis | |
thioredoxin | thioredoxin 1 variant | Bacillus subtilis |