Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression in strain BL21(DE3) as His-tagged protein | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme contains no iron | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl-sulfate + thioredoxin | Escherichia coli | - |
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? | |
additional information | Escherichia coli | enzyme is essential for sulfate assimilation using phosphoadenosine 5'-phosphosulfate, not adenosine 5'-phosphosulfate, phylogenetic comparison of the 2 different groups, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl-sulfate + thioredoxin | - |
Escherichia coli | adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? | |
3'-phosphoadenylyl-sulfate + thioredoxin | thioredoxin m from spinach | Escherichia coli | adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? | |
additional information | enzyme is essential for sulfate assimilation using phosphoadenosine 5'-phosphosulfate, not adenosine 5'-phosphosulfate, phylogenetic comparison of the 2 different groups, overview | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3'-Phosphoadenylylsulfate reductase | - |
Escherichia coli |
PAPS reductase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Escherichia coli |