Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | isozyme PMSRA2 is slightly induced by paraquat, ozone, and high light conditions | Arabidopsis thaliana | |
additional information | isozyme PMSRA3 expression is slightly induced by ozone | Arabidopsis thaliana | |
additional information | isozyme PMSRA4 is highly induced by high light conditions, and slightly by ozone, paraquat, and cercosporin | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
locus At2g18030, isozyme PMSRA5, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21(DE3), prediction of cis-elements in the promoter, overview | Arabidopsis thaliana |
locus At4g25130, isozyme PMSRA4, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of His-tagged isozyme PMSRA4 in Escherichia coli, prediction of cis-elements in the promoter, overview | Arabidopsis thaliana |
locus At5g07460, isozyme PMSRA2, DNA and amino acid sequence determination and analysis, phylogenetic tree, prediction of cis-elements in the promoter, overview | Arabidopsis thaliana |
locus At5g07470, isozyme PMSRA3, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of His-tagged isozyme PMSRA3 in Escherichia coli, prediction of cis-elements in the promoter, overview | Arabidopsis thaliana |
locus At5g61640, isozyme PMSRA1, DNA and amino acid sequence determination and analysis, phylogenetic tree, prediction of cis-elements in the promoter, overview | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | isozyme PMSRA5 expression is slightly suppressed by ozone | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide | recombinant isozyme PMSRA4, with DTT, pH 8.0, 22°C | Arabidopsis thaliana | |
2.12 | - |
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide | recombinant isozyme PMSRA3, with DTT, pH 7.5, 22°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isozyme PMSRA3 | Arabidopsis thaliana | 9507 | - |
chloroplast | isozyme PMSRA4 | Arabidopsis thaliana | 9507 | - |
cytosol | cytosolic isozyme PMsrA1 | Arabidopsis thaliana | 5829 | - |
cytosol | isozyme PMSRA2 | Arabidopsis thaliana | 5829 | - |
additional information | subcellular localization of isozymes, overview | Arabidopsis thaliana | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide-L-methionine-(S)-S-oxide + thioredoxin | Arabidopsis thaliana | stereospecific reduction | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P54150 | isozyme PMSRA1 | - |
Arabidopsis thaliana | P54150 | isozyme PMSRA2 | - |
Arabidopsis thaliana | P54150 | isozyme PMSRA3 | - |
Arabidopsis thaliana | P54150 | isozyme PMSRA4 | - |
Arabidopsis thaliana | Q9SL43 | isozyme PMSRA5 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme PMSRA3 from Escherichia coli by nickel affinity chromatography | Arabidopsis thaliana |
recombinant His-tagged isozyme PMSRA4 from Escherichia coli by nickel affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cauline leaf | - |
Arabidopsis thaliana | - |
cauline leaf | highest expression level of isozyme PMSRA4 | Arabidopsis thaliana | - |
flower | mature, after anthesis | Arabidopsis thaliana | - |
flower bud | - |
Arabidopsis thaliana | - |
leaf | young and mature | Arabidopsis thaliana | - |
additional information | quantitative expression profile analysis | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
root | highest expression level of isozyme PMSRA3 | Arabidopsis thaliana | - |
seedling | germinated | Arabidopsis thaliana | - |
seedling | germinated, highest expression level of isozyme PMSRA4 | Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
stem | highest expression level of isozyme PMSRA2 | Arabidopsis thaliana | - |
stem | highest expression level of isozyme PMSRA5 | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fmoc-L-methionine (S)-sulfoxide + dithiothreitol | - |
Arabidopsis thaliana | Fmoc-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
L-methionine-(S)-S-oxide + DTT | stereospecific reduction, 9-fluorenylmethyl chloroformate-labeled substrate | Arabidopsis thaliana | L-methionine + DTT disulfide + H2O | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | stereospecific reduction | Arabidopsis thaliana | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the peptide methionine sulfoxide reductase A, PMSRA, gene family | Arabidopsis thaliana |
peptide methionine sulfoxide reductase A | - |
Arabidopsis thaliana |
PMSRA | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.91 | - |
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide | recombinant isozyme PMSRA3, with DTT, pH 7.5, 22°C | Arabidopsis thaliana | |
1.59 | - |
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide | recombinant isozyme PMSRA4, with DTT, pH 8.0, 22°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arabidopsis thaliana |
7.5 | 8 | assay at | Arabidopsis thaliana |
8 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Arabidopsis thaliana | |
thioredoxin | - |
Arabidopsis thaliana |