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Literature summary for 1.8.4.11 extracted from

  • Romero, H.M.; Pell, E.J.; Tien, M.
    Expression profile analysis and biochemical properties of the peptide methionine sulfoxide reductase A (PMSRA) gene family in Arabidopsis (2006), Plant Sci., 170, 705-714.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information isozyme PMSRA2 is slightly induced by paraquat, ozone, and high light conditions Arabidopsis thaliana
additional information isozyme PMSRA3 expression is slightly induced by ozone Arabidopsis thaliana
additional information isozyme PMSRA4 is highly induced by high light conditions, and slightly by ozone, paraquat, and cercosporin Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
locus At2g18030, isozyme PMSRA5, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21(DE3), prediction of cis-elements in the promoter, overview Arabidopsis thaliana
locus At4g25130, isozyme PMSRA4, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of His-tagged isozyme PMSRA4 in Escherichia coli, prediction of cis-elements in the promoter, overview Arabidopsis thaliana
locus At5g07460, isozyme PMSRA2, DNA and amino acid sequence determination and analysis, phylogenetic tree, prediction of cis-elements in the promoter, overview Arabidopsis thaliana
locus At5g07470, isozyme PMSRA3, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of His-tagged isozyme PMSRA3 in Escherichia coli, prediction of cis-elements in the promoter, overview Arabidopsis thaliana
locus At5g61640, isozyme PMSRA1, DNA and amino acid sequence determination and analysis, phylogenetic tree, prediction of cis-elements in the promoter, overview Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
additional information isozyme PMSRA5 expression is slightly suppressed by ozone Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide recombinant isozyme PMSRA4, with DTT, pH 8.0, 22°C Arabidopsis thaliana
2.12
-
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide recombinant isozyme PMSRA3, with DTT, pH 7.5, 22°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isozyme PMSRA3 Arabidopsis thaliana 9507
-
chloroplast isozyme PMSRA4 Arabidopsis thaliana 9507
-
cytosol cytosolic isozyme PMsrA1 Arabidopsis thaliana 5829
-
cytosol isozyme PMSRA2 Arabidopsis thaliana 5829
-
additional information subcellular localization of isozymes, overview Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptide-L-methionine-(S)-S-oxide + thioredoxin Arabidopsis thaliana stereospecific reduction peptide-L-methionine + thioredoxin disulfide + H2O
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P54150 isozyme PMSRA1
-
Arabidopsis thaliana P54150 isozyme PMSRA2
-
Arabidopsis thaliana P54150 isozyme PMSRA3
-
Arabidopsis thaliana P54150 isozyme PMSRA4
-
Arabidopsis thaliana Q9SL43 isozyme PMSRA5
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme PMSRA3 from Escherichia coli by nickel affinity chromatography Arabidopsis thaliana
recombinant His-tagged isozyme PMSRA4 from Escherichia coli by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
cauline leaf
-
Arabidopsis thaliana
-
cauline leaf highest expression level of isozyme PMSRA4 Arabidopsis thaliana
-
flower mature, after anthesis Arabidopsis thaliana
-
flower bud
-
Arabidopsis thaliana
-
leaf young and mature Arabidopsis thaliana
-
additional information quantitative expression profile analysis Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
root highest expression level of isozyme PMSRA3 Arabidopsis thaliana
-
seedling germinated Arabidopsis thaliana
-
seedling germinated, highest expression level of isozyme PMSRA4 Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-
stem highest expression level of isozyme PMSRA2 Arabidopsis thaliana
-
stem highest expression level of isozyme PMSRA5 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fmoc-L-methionine (S)-sulfoxide + dithiothreitol
-
Arabidopsis thaliana Fmoc-L-methionine + dithiothreitol disulfide + H2O
-
?
L-methionine-(S)-S-oxide + DTT stereospecific reduction, 9-fluorenylmethyl chloroformate-labeled substrate Arabidopsis thaliana L-methionine + DTT disulfide + H2O
-
?
peptide-L-methionine-(S)-S-oxide + thioredoxin stereospecific reduction Arabidopsis thaliana peptide-L-methionine + thioredoxin disulfide + H2O
-
?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the peptide methionine sulfoxide reductase A, PMSRA, gene family Arabidopsis thaliana
peptide methionine sulfoxide reductase A
-
Arabidopsis thaliana
PMSRA
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.91
-
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide recombinant isozyme PMSRA3, with DTT, pH 7.5, 22°C Arabidopsis thaliana
1.59
-
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide recombinant isozyme PMSRA4, with DTT, pH 8.0, 22°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Arabidopsis thaliana
7.5 8 assay at Arabidopsis thaliana
8
-
assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
dithiothreitol
-
Arabidopsis thaliana
thioredoxin
-
Arabidopsis thaliana