Cloned (Comment) | Organism |
---|---|
gene msrA, expression in strain B834(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
15-50 mg/ml purified recombinant MsrA in 50 mM Tris-HCl, pH 8.0, 2 mM EDTA, and 10 mM DTT, hanging drop vapour diffusion method, droplet size is 0.004-0.008 ml, equal volumes of protein and precipitant solution, X-ray diffraction structure determination and analysis at 1.9 A resolution | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide-L-methionine-(S)-S-oxide + thioredoxin | Escherichia coli | MsrA is involved in the antioxidant defense | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant MsrA from strain B834(DE3) | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide-L-methionine-(S)-S-oxide + DTT | - |
Escherichia coli | peptide-L-methionine + DTT disulfide + H2O | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | - |
Escherichia coli | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | MsrA is involved in the antioxidant defense | Escherichia coli | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MsrA | - |
Escherichia coli |
peptide methionine sulfoxide reductase | - |
Escherichia coli |
PMSR | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Escherichia coli | |
thioredoxin | - |
Escherichia coli |