Cloned (Comment) | Organism |
---|---|
overexpression of MsrA in a yeast strain, expression of MsrA as N-terminally 6His-tagged protein in Escherichia coli strain BL-21 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-sulfoxide + thioredoxin | Saccharomyces cerevisiae | MsrA specifically reduces the S-form of methionine sulfoxide | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | Saccharomyces cerevisiae BY4741 | MsrA specifically reduces the S-form of methionine sulfoxide | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | Saccharomyces cerevisiae | the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview | ? | - |
? | |
additional information | Saccharomyces cerevisiae BY4741 | the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
enzyme MsrA | - |
Saccharomyces cerevisiae BY4741 | - |
enzyme MsrA | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant activity in overexpressing yeast cells | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dabsyl-L-methionine (S)-sulfoxide + thioredoxin | MsrA specifically reduces the S-form of methionine sulfoxide | Saccharomyces cerevisiae | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | MsrA specifically reduces the S-form of methionine sulfoxide | Saccharomyces cerevisiae | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | MsrA specifically reduces the S-form of methionine sulfoxide | Saccharomyces cerevisiae BY4741 | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview | Saccharomyces cerevisiae | ? | - |
? | |
additional information | the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview | Saccharomyces cerevisiae BY4741 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase | - |
Saccharomyces cerevisiae |
MsrA | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Saccharomyces cerevisiae |