Cloned (Comment) | Organism |
---|---|
overexpression of N-terminally 10His-tagged enzyme MsrA in Escherichia coli strain Bl21(DE3) | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
single crystals of recombinant N-terminally 10His-tagged enzyme MsrA complexed with protein-bound methionine, hanging drop method, 30 mg/ml protein in 25 mM Tris-HCl, pH 8.0, 1 mM EDTA, 1 mM tris(carboxyethyl)phosphine hydrochloride, precipitant solution contains 2.0 M sodium formate, 0.1 M sodium citrate, pH 6.0, 4°C, 1 week, prior to data collection, crystals are soaked in 6.3 M sodium formate, 0.1 M sodium citrate, pH 6.0, for 2 min, and are flash-cooled, X-ray diffraction structure determination and analysis at 1.5 A resolution, polycrystalline clusters are obtained by sitting drop vapor diffusion method | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | msrA gene disruption leads to loss of pathogenicity of the bacterium due to loss of ability to colonize host cells in humans | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-sulfoxide + thioredoxin | Mycobacterium tuberculosis | - |
L-methionine + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally 10His-tagged enzyme MsrA from Escherichia coli strain Bl21(DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism of MsrA, active site structure, modeling of protein-bound methionine sulfoxide recognition and repair from the crystal structure | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-sulfoxide + thioredoxin | - |
Mycobacterium tuberculosis | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | absolute specificity for the S-form | Mycobacterium tuberculosis | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | enzyme MsrA, absolute specificity for the S-form | Mycobacterium tuberculosis | L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase A | - |
Mycobacterium tuberculosis |
MsrA | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Mycobacterium tuberculosis |