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Literature summary for 1.8.4.11 extracted from

  • Ezraty, B.; Aussel, L.; Barras, F.
    Methionine sulfoxide reductases in prokaryotes (2005), Biochim. Biophys. Acta, 1703, 221-229.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme is induced under oxidative stress and heat shock Mycobacterium tuberculosis
additional information gene msrA is induced during biofilm formation Escherichia coli
additional information the msrA1-msrB operon is induced by antibiotics, expression of msrA1 is increased in the stationary phase but not affected by H2O2 Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment) Organism
3 genes msrA and 1 gene msrB form an operon, one of the 3 msrA genes is fused to the msrB gene, genetic organization and regulation, overview Staphylococcus aureus
chromosome 1 contains 1 gene msrA, chromosome 2 contains 1 gene msrA Vibrio cholerae serotype O1
gene msrA, located in the chromosome at 95.69 min, respectively, recombinant expression of msrA, regulation mechanism of gene expression, overview Escherichia coli
genes msrA and msrB are translationally fused Neisseria gonorrhoeae
genes msrA and msrB, EC 1.8.4.12, form an operon Bacillus subtilis
genes msrA and msrB, methionine S-oxide reductase (R-form oxidizing), are translationally fused Neisseria meningitidis
the chromosome contains 2 copies of gene msrA, a plasmid harbors 1 copy of gene msrA Sinorhizobium meliloti

Protein Variants

Protein Variants Comment Organism
additional information a msrA mutant exhibited reduced adherence to erythrocytes as well as increased sensitivity to H2O2 and tert-butyl hydroperoxide killing, the mutant is not able to survive in hamster lungs Mycoplasma genitalium
additional information construction of a msrA mutant which shows drastically reduced ability to survive in macrophages compared to the wild-type strain, the mutant shows increased sensitivity to cumene hydroperoxide and tert-butyl hydroperoxide, but not to H2O2, paraquat, or sodium nitrite Mycolicibacterium smegmatis
additional information construction of several mutants with reduced virulence via transposon mutagenesis involving mutation of msrA, mutation of msrA leads o increased sensitivity to oxidative agents, non-motility, and reduced spreading-out and life-span of the pathogen in plants Dickeya chrysanthemi
additional information mutant strains produce a truncated version of fused MsrA/MsrB with increased sensitivity to H2O2 and superoxide anions Neisseria gonorrhoeae
additional information mutation of gene msrA has no effect on virulence, and on resistance to oxidative agents, and causes no defect in cell envelope, msrA is probably linked to biofilm formation Aggregatibacter actinomycetemcomitans
additional information mutation of msrA results in reduced development of mature biofilm Escherichia coli
additional information mutation of the msr genes impair virulence, overview, mutation of the msrA1 operon leads to increased susceptibility to H2O2 Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion of MsrA and MsrB, methionine S-oxide reductase (R-form oxidizing), fused together Helicobacter pylori
-
-
membrane outer cell membrane Neisseria gonorrhoeae 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine (S)-sulfoxide + thioredoxin Staphylococcus aureus MsrA is specific for the S-form L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin Mycoplasma genitalium MsrA is specific for the S-form L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin Sinorhizobium meliloti MsrA is specific for the S-form L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin Bacillus subtilis MsrA is specific for the S-form L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin Escherichia coli MsrA is specific for the S-form L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin Mycolicibacterium smegmatis MsrA is specific for the S-form L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin Neisseria gonorrhoeae MsrA is specific for the S-form L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin Neisseria meningitidis MsrA is specific for the S-form L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin Mycobacterium tuberculosis MsrA is specific for the S-form L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin Helicobacter pylori MsrA is specific for the S-form L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin Dickeya chrysanthemi MsrA is specific for the S-form L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin Vibrio cholerae serotype O1 MsrA is specific for the S-form L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin Aggregatibacter actinomycetemcomitans MsrA is specific for the S-form L-methionine + thioredoxin disulfide + H2O
-
?
additional information Neisseria gonorrhoeae enzyme acts on free and protein-bound methionine ?
-
?
additional information Mycoplasma genitalium enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide ?
-
?
additional information Mycobacterium tuberculosis enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, e.g. the heat shock protein and chaperone Hsp16.3, role of the MsrA/MsrB repair pathway in cellular protein dynamics ?
-
?
additional information Sinorhizobium meliloti enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics ?
-
?
additional information Bacillus subtilis enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics ?
-
?
additional information Mycolicibacterium smegmatis enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics ?
-
?
additional information Helicobacter pylori enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics ?
-
?
additional information Dickeya chrysanthemi enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics ?
-
?
additional information Vibrio cholerae serotype O1 enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics ?
-
?
additional information Staphylococcus aureus enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics, MsrA is important for virulence in mice ?
-
?
additional information Escherichia coli enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics, the MsrA/MsrB repair pathway is involved in the signal recognition particle-dependent protein targeting pathway, regulation mechanism of gene expression, overview ?
-
?
additional information Neisseria meningitidis enzymes acts on free and protein-bound methionine ?
-
?
additional information Aggregatibacter actinomycetemcomitans role of the MsrA/MsrB repair pathway in cellular protein dynamics, mutation of gene msrA has no effect on virulence, and on resistance to oxidative agents, and causes no defect in cell envelope, msrA is probably linked to biofilm formation, enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide ?
-
?

Organism

Organism UniProt Comment Textmining
Aggregatibacter actinomycetemcomitans
-
-
-
Bacillus subtilis
-
-
-
Dickeya chrysanthemi
-
-
-
Escherichia coli
-
1 copy gene msrA
-
Helicobacter pylori
-
-
-
Mycobacterium tuberculosis
-
-
-
Mycolicibacterium smegmatis
-
-
-
Mycoplasma genitalium
-
-
-
Neisseria gonorrhoeae
-
-
-
Neisseria meningitidis
-
-
-
no activity in Aquifex aeolicus
-
-
-
no activity in Thermotoga maritima
-
-
-
Sinorhizobium meliloti
-
3 copies of gene msrA
-
Staphylococcus aureus
-
3 copies of gene msrA, encoding MsrA1, MsrA2,and MsrA3
-
Vibrio cholerae serotype O1
-
2 isozymes of MsrA
-

Reaction

Reaction Comment Organism Reaction ID
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O catalytic mechanism involves the formation of a sulfenic acid intermediate Staphylococcus aureus
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O catalytic mechanism involves the formation of a sulfenic acid intermediate Mycoplasma genitalium
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O catalytic mechanism involves the formation of a sulfenic acid intermediate Sinorhizobium meliloti
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O catalytic mechanism involves the formation of a sulfenic acid intermediate Bacillus subtilis
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O catalytic mechanism involves the formation of a sulfenic acid intermediate Escherichia coli
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O catalytic mechanism involves the formation of a sulfenic acid intermediate Mycolicibacterium smegmatis
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O catalytic mechanism involves the formation of a sulfenic acid intermediate Neisseria gonorrhoeae
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O catalytic mechanism involves the formation of a sulfenic acid intermediate Neisseria meningitidis
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O catalytic mechanism involves the formation of a sulfenic acid intermediate Mycobacterium tuberculosis
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O catalytic mechanism involves the formation of a sulfenic acid intermediate Helicobacter pylori
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O catalytic mechanism involves the formation of a sulfenic acid intermediate Dickeya chrysanthemi
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O catalytic mechanism involves the formation of a sulfenic acid intermediate Vibrio cholerae serotype O1
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O catalytic mechanism involves the formation of a sulfenic acid intermediate Aggregatibacter actinomycetemcomitans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine (S)-sulfoxide + thioredoxin MsrA is specific for the S-form Staphylococcus aureus L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin MsrA is specific for the S-form Mycoplasma genitalium L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin MsrA is specific for the S-form Sinorhizobium meliloti L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin MsrA is specific for the S-form Bacillus subtilis L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin MsrA is specific for the S-form Escherichia coli L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin MsrA is specific for the S-form Mycolicibacterium smegmatis L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin MsrA is specific for the S-form Neisseria gonorrhoeae L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin MsrA is specific for the S-form Neisseria meningitidis L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin MsrA is specific for the S-form Mycobacterium tuberculosis L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin MsrA is specific for the S-form Helicobacter pylori L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin MsrA is specific for the S-form Dickeya chrysanthemi L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin MsrA is specific for the S-form Vibrio cholerae serotype O1 L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin MsrA is specific for the S-form Aggregatibacter actinomycetemcomitans L-methionine + thioredoxin disulfide + H2O
-
?
additional information enzyme acts on free and protein-bound methionine Staphylococcus aureus ?
-
?
additional information enzyme acts on free and protein-bound methionine Mycoplasma genitalium ?
-
?
additional information enzyme acts on free and protein-bound methionine Sinorhizobium meliloti ?
-
?
additional information enzyme acts on free and protein-bound methionine Bacillus subtilis ?
-
?
additional information enzyme acts on free and protein-bound methionine Escherichia coli ?
-
?
additional information enzyme acts on free and protein-bound methionine Mycolicibacterium smegmatis ?
-
?
additional information enzyme acts on free and protein-bound methionine Neisseria gonorrhoeae ?
-
?
additional information enzyme acts on free and protein-bound methionine Neisseria meningitidis ?
-
?
additional information enzyme acts on free and protein-bound methionine Mycobacterium tuberculosis ?
-
?
additional information enzyme acts on free and protein-bound methionine Helicobacter pylori ?
-
?
additional information enzyme acts on free and protein-bound methionine Dickeya chrysanthemi ?
-
?
additional information enzyme acts on free and protein-bound methionine Vibrio cholerae serotype O1 ?
-
?
additional information enzyme acts on free and protein-bound methionine Aggregatibacter actinomycetemcomitans ?
-
?
additional information enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide Mycoplasma genitalium ?
-
?
additional information enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, e.g. the heat shock protein and chaperone Hsp16.3, role of the MsrA/MsrB repair pathway in cellular protein dynamics Mycobacterium tuberculosis ?
-
?
additional information enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics Sinorhizobium meliloti ?
-
?
additional information enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics Bacillus subtilis ?
-
?
additional information enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics Mycolicibacterium smegmatis ?
-
?
additional information enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics Helicobacter pylori ?
-
?
additional information enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics Dickeya chrysanthemi ?
-
?
additional information enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics Vibrio cholerae serotype O1 ?
-
?
additional information enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics, MsrA is important for virulence in mice Staphylococcus aureus ?
-
?
additional information enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics, the MsrA/MsrB repair pathway is involved in the signal recognition particle-dependent protein targeting pathway, regulation mechanism of gene expression, overview Escherichia coli ?
-
?
additional information enzymes acts on free and protein-bound methionine Neisseria meningitidis ?
-
?
additional information role of the MsrA/MsrB repair pathway in cellular protein dynamics, mutation of gene msrA has no effect on virulence, and on resistance to oxidative agents, and causes no defect in cell envelope, msrA is probably linked to biofilm formation, enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide Aggregatibacter actinomycetemcomitans ?
-
?

Subunits

Subunits Comment Organism
More MsrA and enzyme MsrB, methionine S-oxide reductase (R-form oxidizing), form domains of a single polypeptide together with a third thioredoxin-like domain Neisseria meningitidis
More MsrA and MsrB, EC 1.8.4.12, are fused together Helicobacter pylori
More the 2 enzyme activities, MsrA and MsrB, form domains of a single polypeptide together with a third thioredoxin-like domain Neisseria gonorrhoeae

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase
-
Staphylococcus aureus
methionine sulfoxide reductase
-
Mycoplasma genitalium
methionine sulfoxide reductase
-
Sinorhizobium meliloti
methionine sulfoxide reductase
-
Bacillus subtilis
methionine sulfoxide reductase
-
Escherichia coli
methionine sulfoxide reductase
-
Mycolicibacterium smegmatis
methionine sulfoxide reductase
-
Neisseria gonorrhoeae
methionine sulfoxide reductase
-
Neisseria meningitidis
methionine sulfoxide reductase
-
Mycobacterium tuberculosis
methionine sulfoxide reductase
-
Helicobacter pylori
methionine sulfoxide reductase
-
Dickeya chrysanthemi
methionine sulfoxide reductase
-
Vibrio cholerae serotype O1
methionine sulfoxide reductase
-
Aggregatibacter actinomycetemcomitans
MsrA
-
Staphylococcus aureus
MsrA
-
Mycoplasma genitalium
MsrA
-
Sinorhizobium meliloti
MsrA
-
Bacillus subtilis
MsrA
-
Escherichia coli
MsrA
-
Mycolicibacterium smegmatis
MsrA
-
Neisseria gonorrhoeae
MsrA
-
Neisseria meningitidis
MsrA
-
Mycobacterium tuberculosis
MsrA
-
Helicobacter pylori
MsrA
-
Dickeya chrysanthemi
MsrA
-
Vibrio cholerae serotype O1
MsrA
-
Aggregatibacter actinomycetemcomitans

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
Staphylococcus aureus
thioredoxin
-
Mycoplasma genitalium
thioredoxin
-
Sinorhizobium meliloti
thioredoxin
-
Bacillus subtilis
thioredoxin
-
Escherichia coli
thioredoxin
-
Mycolicibacterium smegmatis
thioredoxin
-
Neisseria gonorrhoeae
thioredoxin
-
Neisseria meningitidis
thioredoxin
-
Mycobacterium tuberculosis
thioredoxin
-
Helicobacter pylori
thioredoxin
-
Dickeya chrysanthemi
thioredoxin
-
Vibrio cholerae serotype O1
thioredoxin
-
Aggregatibacter actinomycetemcomitans