Cloned (Comment) | Organism |
---|---|
mrsA1, and msrA2 are transcribed as polycistronic transcript, overexpression of MsrA1, and MsrA2 as His-tagged proteins in Escherichia coliBL21(DE3) | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
MsrA, which appears in 2 enzyme forms | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged MsrA1, and MsrA2 from Escherichia coli by nickel affinity chromatography | Staphylococcus aureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.063 | - |
purified recombinant MsrA1, substrate L-methionine (S)-sulfoxide | Staphylococcus aureus |
0.24 | - |
purified recombinant MsrA2, substrate L-methionine (S)-sulfoxide | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-sulfoxide + thioredoxin | the 2 MsrA enzymes are absolutely specific for the S-form of the substrate | Staphylococcus aureus | L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase | - |
Staphylococcus aureus |
MsrA | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Staphylococcus aureus |