Cloned (Comment) | Organism |
---|---|
gene msrA, expression in Escherichia coli strain BL21(DE3) as N-terminally His-tagged enzyme, the HIs-tag does not influence enzyme activity | Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.18 | - |
dabsyl L-methionine-(S)-sulfoxide | pH 7.4, 37°C, recombinant enzyme | Caenorhabditis elegans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29700 | - |
x * 29700, recombinant MsrA, SDS-PAGE | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | O02089 | gene msrA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged MsrA from Escherichia coli strain BL21(DE3) by nickel affinity and ion exchange chromatography, 425fold | Caenorhabditis elegans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.96 | - |
purified enzyme, substrate dabsyl L-methionine-(S)-sulfoxide with dithiothreitol | Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dabsyl-L-methionine (S)-sulfoxide + dithiothreitol | synthetic substrate, MsrA is absolutely specific for the S-form | Caenorhabditis elegans | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
dabsyl-L-methionine (S)-sulfoxide + NADPH + H+ | synthetic substrate, MsrA is absolutely specific for the S-form, 7fold lower activity with NADPH compared to DTT | Caenorhabditis elegans | dabsyl-L-methionine + NADP+ + H2O | - |
? | |
dabsyl-L-methionine (S)-sulfoxide + thioredoxin | synthetic substrate, MsrA is absolutely specific for the S-form | Caenorhabditis elegans | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | enzyme converts free and protein-bound methionine | Caenorhabditis elegans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29700, recombinant MsrA, SDS-PAGE | Caenorhabditis elegans |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide-S-reductase | - |
Caenorhabditis elegans |
MsrA | - |
Caenorhabditis elegans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
about | Caenorhabditis elegans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
17 | 57 | activity rapidly decreases below 17°C and above 57°C | Caenorhabditis elegans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
dabsyl L-methionine-(S)-sulfoxide | pH 7.4, 37°C, recombinant enzyme | Caenorhabditis elegans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Caenorhabditis elegans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | sharp decrease below pH 6.5 and above pH 8.5 | Caenorhabditis elegans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | preferred cofactor in vitro | Caenorhabditis elegans | |
NADPH | - |
Caenorhabditis elegans | |
thioredoxin | - |
Caenorhabditis elegans |