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Literature summary for 1.8.4.11 extracted from
- Repair of oxidized proteins: identification of a new methionine sulfoxide reductase (2001), J. Biol. Chem., 276, 48915-48920.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene msrA, expression in Escherichia coli strain BL21(DE3) as His-tagged enzyme | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-methionine (S)-sulfoxide + thioredoxin | Escherichia coli | enzyme is involved in repairing of oxidized methionine residues in proteins | L-methionine + thioredoxin disulfide + H2O | - |
? |  |
| additional information | Escherichia coli | physiological role | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene msrA, enzyme is organized in a fusion protein together with MsrB, EC 1.8.4.B1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.33 | - |
in vitro, substrate free L-methionine (R)-sulfoxide | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-methionine (S)-sulfoxide + thioredoxin | enzyme is involved in repairing of oxidized methionine residues in proteins | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (S)-sulfoxide + thioredoxin | MsrA is specific for the S-form of the substrate | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| additional information | physiological role | Escherichia coli | ? | - |
? | |
| oxidized calmodulin + thioredoxin | enzyme reduces L-methionine (S)-sulfoxide of the protein substrate | Escherichia coli | partially reduced calmodulin + thioredoxin disulfide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methionine sulfoxide reductase | - |
Escherichia coli |
| MsrA | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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