Literature summary for 1.8.1.4 extracted from

Gutierrez-Correa, J.; Krauth-Siegel, R.L.; Stoppani, A.O.
Phenothiazine radicals inactivate Trypanosoma cruzi dihydrolipoamide dehydrogenase: enzyme protection by radical scavengers (2003), Free Radic. Res., 37, 281-291.

Search for more literature for 1.8.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain JRG 1342	Trypanosoma cruzi

Inhibitors

Inhibitors	Comment	Organism	Structure
10-(2-dimethylaminopropyl)-dibenzothiazine cation radical	60% inactivation after 10 min incubation and 79% after 30 min using the myeloperoxidase system, 72% inactivation after 10 min incubation using the horseradish peroxidase system	Trypanosoma cruzi
10-(2-methyl,3-dimethylaminopropyl)-dibenzothiazine cation radical	90% inactivation after 10 min and 30 min incubation using the myeloperoxidase system, 94% inactivation after 10 min incubation using the horseradish peroxidase system	Trypanosoma cruzi
10-(3-dimethylaminopropyl)-dibenzothiazine cation radical	87% inactivation after 10 min incubation and 89% after 30 min using the myeloperoxidase system, 94% inactivation after 10 min incubation using the horseradish peroxidase system	Trypanosoma cruzi
2-chloro-10-(3-dimethylaminopropyl)-dibenzothiazine cation radical	45% inactivation after 10 min incubation and 75% after 30 min using the myeloperoxidase system, 89% inactivation after 10 min incubation using the horseradish peroxidase system	Trypanosoma cruzi
2-chloro-10-[3-(1-methyl-4-piperazinyl)-propyl]-dibenzothiazine cation radical	54% inactivation after 10 min incubation and 80% after 30 min using the myeloperoxidase system, 90% inactivation after 10 min incubation using the horseradish peroxidase system	Trypanosoma cruzi
2-chloro-10-[3-[1-(2-hydroxyethyl)-4-piperazinyl]propyl]-dibenzothiazine cation radical	42% inactivation after 10 min incubation and 69% after 30 min using the myeloperoxidase system, 79% inactivation after 10 min incubation using the horseradish peroxidase system	Trypanosoma cruzi
2-methylmercapto-10-[2-(1-methyl-2-piperidinyl)-ethyl]-dibenzothiazine cation radical	77% inactivation after 10 min incubation and 82% after 30 min using the myeloperoxidase system, 85% inactivation after 10 min incubation using the horseradish peroxidase system	Trypanosoma cruzi
2-propionyl-10-(3-dimethylaminopropyl)-dibenzothiazine cation radical	11% inactivation after 10 min incubation and 32% after 30 min using the myeloperoxidase system, 83% inactivation after 10 min incubation using the horseradish peroxidase system	Trypanosoma cruzi
2-trifluoromethyl-10-[3-(1-methyl-4-piperazinyl)propyl]-dibenzothiazine cation radical	5% inactivation after 10 min incubation and 16% after 30 min using the myeloperoxidase system, 67% inactivation after 10 min incubation using the horseradish peroxidase system	Trypanosoma cruzi
2-trifluoromethyl-10-[3-(dimethylamino)propyl]-dibenzothiazine cation radical	2% inactivation after 10 and 30 min incubation using the myeloperoxidase system, 16% inactivation after 10 min incubation using the horseradish peroxidase system	Trypanosoma cruzi
2-trifluoromethyl-10-[3-[1-(2-hydroxyethyl)4-piperazinyl]propyl]-dibenzothiazine cation radical	1% inactivation after 10 min incubation and 8% after 30 min using the myeloperoxidase system, 61% inactivation after 10 min incubation using the horseradish peroxidase system	Trypanosoma cruzi
additional information	no inhibition by the cation radical of 2-cyano-10-[3-(4-hydroxypiperidinyl)propyl]-dibenzothiazine in either peroxidase system	Trypanosoma cruzi
phenothiazine cation radicals	irreversible inactivation dependent on time, radical structure, and radical production enzyme system, radicals are produced by reaction of myeloperoxidase or horse radish peroxidase on the phenothiazines promazine, trimeprazine, thioridazine, chlorpromazine, prochlorperazine, promethazine, and others, in presence of H2O2, protection by radical scavengers e.g. thiol compounds, amino acids and peptides, pyridine dinucleotides like NADH, or best by ascorbate and trolox, overview	Trypanosoma cruzi

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
400	-	about, purified recombinant enzyme	Trypanosoma cruzi

Storage Stability

Storage Stability	Organism
purified recombinant enzyme, -20°C, 100 mM potassium phosphate, 100 mM KCl, pH 7.0, 1 mM EDTA, 50% v/v glycerol, stable for at least 1 year	Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
lipoamide + NADH	-	Trypanosoma cruzi	dihydrolipoamide + NAD+	-	?

Synonyms

Synonyms	Comment	Organism
dihydrolipoamide dehydrogenase	-	Trypanosoma cruzi
LADH	-	Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Trypanosoma cruzi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Trypanosoma cruzi

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Trypanosoma cruzi

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)