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Literature summary for 1.7.3.3 extracted from

  • Liu, X.; Wen, M.; Li, J.; Zhai, F.; Ruan, J.; Zhang, L.; Li, S.
    High-yield expression, purification, characterization, and structure determination of tag-free Candida utilis uricase (2011), Appl. Microbiol. Biotechnol., 92, 529-537.
    View publication on PubMed

Application

Application Comment Organism
synthesis high-yield expression of uricase in Escherichia coli and establishment of an efficient three-step protein purification protocol. The purity of the recombinant protein is more than 98% and the specific activity is 38.4 IU/mg Cyberlindnera jadinii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cyberlindnera jadinii

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.93 A resolution. Space group P212121 with unit cell parameters a 69.16 A, b 139.31 A, c 256.33 A, and alpha =beta =gamma =90° Cyberlindnera jadinii

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 2 mM, complete inactivation Cyberlindnera jadinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0337
-
Urate pH 8.0, 37°C Cyberlindnera jadinii

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 2 mM, 170% of initial activity Cyberlindnera jadinii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34160
-
x * 34160, MALDI-TOF Cyberlindnera jadinii

Organism

Organism UniProt Comment Textmining
Cyberlindnera jadinii P78609
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
38.4
-
recombinant protein, pH 8.0, 37°C Cyberlindnera jadinii

Storage Stability

Storage Stability Organism
-20°C, 72 h, no loss of activity Cyberlindnera jadinii
4°C, 72 h, no loss of activity Cyberlindnera jadinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
Cyberlindnera jadinii 5-hydroxyisourate + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 34160, MALDI-TOF Cyberlindnera jadinii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Cyberlindnera jadinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Cyberlindnera jadinii