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Literature summary for 1.7.3.3 extracted from

  • Kai, L.; Ma, X.; Zhou, X.; Jia, X.; Li, X.; Guo, K.
    Purification and characterization of a thermostable uricase from Microbacterium sp. strain ZZJ4-1 (2008), World J. Microbiol. Biotechnol., 24, 401-406.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 95% inhibition at 1 mM Microbacterium sp.
Hg2+ 98% inhibition at 1 mM Microbacterium sp.
Li+ 18.5% inhibition at 1 mM Microbacterium sp.
additional information no inhibition by Cu2+, Fe3+, or Zn2+ Microbacterium sp.
o-phenanthroline 99% inhibition at 2 mM Microbacterium sp.
SDS 49% inhibition at 0.5% w/v Microbacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
Urate pH 8.5, 30°C Microbacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ activates Microbacterium sp.
additional information a metal ion is possibly strongly bound in the enzyme and forms part of the uricase structure Microbacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000, SDS-PAGE Microbacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urate + O2 + H2O Microbacterium sp.
-
5-hydroxyisourate + H2O2
-
?
urate + O2 + H2O Microbacterium sp. ZZJ4-1
-
5-hydroxyisourate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Microbacterium sp.
-
-
-
Microbacterium sp. ZZJ4-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 19.7fold by ammonium sulfate fractionation, anion exchange exchange and hydrophobic interaction chromatography, and gel filtration Microbacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.32
-
purified enzyme Microbacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
Microbacterium sp. 5-hydroxyisourate + H2O2
-
?
urate + O2 + H2O
-
Microbacterium sp. ZZJ4-1 5-hydroxyisourate + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Microbacterium sp.

Synonyms

Synonyms Comment Organism
urate oxidoreductase
-
Microbacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Microbacterium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 55 20°C: about 80% of maximal activity, maximal activity at 30°C, 55°C: about 55% of maximal activity Microbacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the molecular structure of enzyme has a reversible change at a temperature between 30°C and 60°C Microbacterium sp.
65
-
30 min, the purified enzyme retains 98.9% of its original activity Microbacterium sp.
70
-
30 min, the purified enzyme retains 64% of its original activity Microbacterium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.01
-
Urate pH 8.5, 30°C Microbacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Microbacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 11 pH 6.5: about 40% of maximal activity, pH 10.0: about 50% of maximal activity Microbacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
25°C, 18 h, about 60% loss of activity Microbacterium sp.
7 10 purified enzyme, 25°C, 18 h, stable Microbacterium sp.
11
-
25°C, 18 h, about 60% loss of activity Microbacterium sp.