Application | Comment | Organism |
---|---|---|
drug development | urate oxidase has the potential to be a therapeutic target for the treatment of gout | Cyberlindnera jadinii |
Cloned (Comment) | Organism |
---|---|
developing of a method for genetically incorporating p-azido-L-phenylalanine into target protein in Escherichia coli in a site-specific manner utilizing a tyrosyl suppressor tRNA/aminoacyl-tRNA synthetase system, substitution of p-azido-L-phenylalanine for F170 or F281 in urate oxidase, optimization of the system by adding a Shine-Dalgarno sequence and tandem suppressor tRNA in order to increase the expression levels of tyrosyl suppressor tRNA and aminoacyl-tRNA synthetase | Cyberlindnera jadinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cyberlindnera jadinii | purine degradation, urate oxidase catalyzes the oxidation of uric acid with poor solubility to produce 5-hydroxyisourate and allantoin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyberlindnera jadinii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Ni-NTA affinity chromatography | Cyberlindnera jadinii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.49 | - |
urate oxidase including p-azido-L-phenylalanine instead of Phe at position 281, in 0.1 M borate, pH 8.4 | Cyberlindnera jadinii |
8.26 | - |
urate oxidase including p-azido-L-phenylalanine instead of Phe at position 170, in 0.1 M borate, pH 8.4 | Cyberlindnera jadinii |
16.33 | - |
natural uricase, in 0.1 M borate, pH 8.4 | Cyberlindnera jadinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | purine degradation, urate oxidase catalyzes the oxidation of uric acid with poor solubility to produce 5-hydroxyisourate and allantoin | Cyberlindnera jadinii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Urate oxidase | - |
Cyberlindnera jadinii |
uricase | - |
Cyberlindnera jadinii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Cyberlindnera jadinii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay at | Cyberlindnera jadinii |