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Literature summary for 1.7.3.3 extracted from

  • Lotfy, W.
    Production of a thermostable uricase by a novel Bacillus thermocatenulatus strain (2008), Biores. Technol., 99, 699-702.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
uric acid uric acid (0.3%) is an inducer for uricase production, concentrations higher than 0.3% do not enhance the enzyme productivity Geobacillus thermocatenulatus

Application

Application Comment Organism
medicine determination of the urate concentration in blood and urine is required for the diagnosis of gout as urate accumulation is a causative factor of gout in humans Geobacillus thermocatenulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
uric acid 0.1 M sodium borate, pH 8.5 containing 2 mM uric acid, 30 mM 4-aminoantipyrine, 1.5% phenol and peroxidase (15 U/ml), 37°C, 20 min Geobacillus thermocatenulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urate + O2 + H2O Geobacillus thermocatenulatus ureide pathway 5-hydroxyisourate + H2O2
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?

Organism

Organism UniProt Comment Textmining
Geobacillus thermocatenulatus DQ887577 partial sequence
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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enzyme activity is 0.06 U/ml at pH 4.0 Geobacillus thermocatenulatus
additional information
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enzyme activity is 0.09 U/ml at pH 4.5 Geobacillus thermocatenulatus
additional information
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enzyme activity is 0.11 U/ml at pH 5.0 Geobacillus thermocatenulatus
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enzyme activity is 0.21 U/ml with potassium nitrate as nitrogen source Geobacillus thermocatenulatus
additional information
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enzyme activity is 0.22 U/ml at pH 10.0 Geobacillus thermocatenulatus
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enzyme activity is 0.31 U/ml with ammonium sulfate as nitrogen source Geobacillus thermocatenulatus
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enzyme activity is 0.32 U/ml with sodium glutamate as nitrogen source Geobacillus thermocatenulatus
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enzyme activity is 0.37 U/ml at pH 5.5 Geobacillus thermocatenulatus
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enzyme activity is 0.42 U/ml at pH 9.5 Geobacillus thermocatenulatus
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enzyme activity is 0.45 U/ml with citric acid as carbon source Geobacillus thermocatenulatus
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enzyme activity is 0.46 U/ml with glucose as carbon source Geobacillus thermocatenulatus
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enzyme activity is 0.46 U/ml with peptone as nitrogen source Geobacillus thermocatenulatus
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enzyme activity is 0.47 U/ml at pH 6.0 Geobacillus thermocatenulatus
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enzyme activity is 0.47 U/ml with lactose as carbon source Geobacillus thermocatenulatus
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enzyme activity is 0.49 U/ml with starch as carbon source Geobacillus thermocatenulatus
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enzyme activity is 0.57 U/ml at pH 9.0 Geobacillus thermocatenulatus
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enzyme activity is 0.57 U/ml with soybean flour as nitrogen source Geobacillus thermocatenulatus
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enzyme activity is 0.63 U/ml at pH 8.5 Geobacillus thermocatenulatus
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enzyme activity is 0.65 U/ml with beef extract as nitrogen source Geobacillus thermocatenulatus
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enzyme activity is 0.67 U/ml with sucrose as carbon source Geobacillus thermocatenulatus
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enzyme activity is 0.69 U/ml with yeast extract as nitrogen source Geobacillus thermocatenulatus
additional information
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enzyme activity is 0.72 U/ml at pH 8.0 Geobacillus thermocatenulatus
additional information
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enzyme activity is 0.78 U/ml with maize milk as nitrogen source Geobacillus thermocatenulatus
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enzyme activity is 0.98 U/ml at pH 6.5 Geobacillus thermocatenulatus
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enzyme activity is 1.00 U/ml at pH 7.5 Geobacillus thermocatenulatus
additional information
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enzyme activity is 1.25 U/ml at pH 7.0 Geobacillus thermocatenulatus
additional information
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when the strain is cultured at 30°C at pH 7.0 for 30-36 h with of 0.6% corn steep liquor as nitrogen source, the uricase activity peaks at 1.25 U/ml Geobacillus thermocatenulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O ureide pathway Geobacillus thermocatenulatus 5-hydroxyisourate + H2O2
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?

Synonyms

Synonyms Comment Organism
Urate oxidase
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Geobacillus thermocatenulatus
uricase
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Geobacillus thermocatenulatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
enzyme is thermostable, after heat treatment at 75°C for 45 min, the uricase retains about 100% of its initial activity Geobacillus thermocatenulatus
80
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70% of initial activity remains after 45 min at 80°C Geobacillus thermocatenulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
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optimal pH for uricase production in culture flasks Geobacillus thermocatenulatus
8.5
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assay at Geobacillus thermocatenulatus