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Literature summary for 1.7.3.3 extracted from

  • Pession, A.; Melchionda, F.; Castellini, C.
    Pitfalls, prevention, and treatment of hyperuricemia during tumor lysis syndrome in the era of rasburicase (recombinant urate oxidase) (2008), Biologics, 2, 129-141.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine treatment od tumor lysis syndrome, recombinant urate oxidase is effective in reducing uric acid and preventing uric acid accumulation in patients with hematologic malignancies with hyperuricemia or at high risk of developing it, rasburicase represents an effective alternative to allopurinol to promptly reduce uric acid levels, improve patients electrolyte status, and reverse renal insufficiency Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
using the recombinant DNA technique, enzyme is obtained from a genetically modified Saccharomyces cerevisiae strain that expresses urate oxidase cDNA, cloned from a strain of Aspergillus flavus Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae rasburicase acts at the end of the purine catabolic pathway, and unlike allopurinol, it does not induce accumulation of xanthine or hypoxanthine ?
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?
urate + O2 + H2O Saccharomyces cerevisiae
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5-hydroxyisourate + H2O2 conversion to allantoin ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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-
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
rasburicase causes enzymatic degradation of uric acid within blood, plasma and serum samples at room temperature, the genetic absence of this molecule in humans and its proteic nature together with poor accuracy in purifi cation and a slow production process confer a high immunogenicity to the compound, leading to elevated rate of hypersensivity reactions Saccharomyces cerevisiae
additional information
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rasburicase does not interact with allopurinol, cytarabine, methylprednisolone, methotrexate, mercaptopurine, thioguanine, etoposide, daunorubicin, cyclophosphamide, or vincristine Saccharomyces cerevisiae
additional information
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rasburicase maintains the same mechanism of action as the non-recombinant form of urate oxidase, but simply shows a significantly lower reaction rate Saccharomyces cerevisiae
additional information
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repeated use of rasburicase increases risk of hypersensitivity reactions: skin rashes (1.4%), urticaria, bronchospasm (1%), dyspnea, hypoxemia, and anaphylactic shock (1%) Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information rasburicase acts at the end of the purine catabolic pathway, and unlike allopurinol, it does not induce accumulation of xanthine or hypoxanthine Saccharomyces cerevisiae ?
-
?
urate + O2 + H2O
-
Saccharomyces cerevisiae 5-hydroxyisourate + H2O2 conversion to allantoin ?

Synonyms

Synonyms Comment Organism
Rasburicase recombinant enzyme Saccharomyces cerevisiae
Urate oxidase
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Saccharomyces cerevisiae