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Literature summary for 1.7.3.3 extracted from

  • Li, J.; Chen, Z.; Hou, L.; Fan, H.; Weng, S.; Xu, C.; Ren, J.; Li, B.; Chen, W.
    High-level expression, purification, and characterization of non-tagged Aspergillus flavus urate oxidase in Escherichia coli (2006), Protein Expr. Purif., 49, 55-59.
    View publication on PubMed
Search for more literature for 1.7.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Aspergillus flavus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 SDS-PAGE Aspergillus flavus

Organism

Organism UniProt Comment Textmining
Aspergillus flavus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sepharose FF chromatography, Phenyl-Sepharose FF chromatography and HiLoad 26/60 Superdex 75 gel filtration Aspergillus flavus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.5
-
 in the cell lysate Aspergillus flavus
21.5
-
 after DEAE Sepharose FF chromatography Aspergillus flavus
25.7
-
 after Phenyl-Sepharose FF chromatography Aspergillus flavus
27
-
 after HiLoad 26/60 Superdex 75 gel filtration Aspergillus flavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
 Aspergillus flavus 5-hydroxyisourate + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
Uox
-
 Aspergillus flavus
uricase
-
 Aspergillus flavus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 37
-
 Aspergillus flavus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Aspergillus flavus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus flavus determined by means of P/ACE 5000
-
 8.46