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Literature summary for 1.7.3.3 extracted from

  • Schiavon, O.; Caliceti, P.; Ferruti, P.; Veronese, F.M.
    Therapeutic proteins: a comparison of chemical and biological properties of uricase conjugated to linear or branched poly(ethylene glycol) and poly(N-acryloylmorpholine) (2000), Farmaco, 55, 264-269.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information uricase covalently linked to monomethoxypoly(ethylene glycol) N-leucine-OSu, branched monomethoxypoly(ethylene glycol) N-leucine-OSu or poly(N-acryloylmorpholine)-OSu last longer in blood Metabacillus fastidiosus

General Stability

General Stability Organism
conjugated uricases are more stable to trypsin digestion Metabacillus fastidiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
uric acid poly(N-acryloylmorpholine)-OSu-uricase Metabacillus fastidiosus
0.05
-
uric acid native uricase, monomethoxypoly(ethylene glycol) N-leucine-OSu-uricase and branched monomethoxypoly(ethylene glycol) N-leucine-OSu-uricase Metabacillus fastidiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urate + O2 + H2O Metabacillus fastidiosus
-
5-hydroxyisourate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Metabacillus fastidiosus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
Metabacillus fastidiosus 5-hydroxyisourate + H2O2
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
uricase covalently linked to monomethoxypoly(ethylene glycol) N-leucine-OSu-uricase and branched monomethoxypoly(ethylene glycol) N-leucine-OSu-uricase 50% loss of activity Metabacillus fastidiosus
60 61 native uricase and poly(N-acryloylmorpholine)-OSu-uricase 50% loss of activity Metabacillus fastidiosus