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Literature summary for 1.7.2.4 extracted from

  • Wan, S.; Mottiar, Y.; Johnson, A.M.; Goto, K.; Altosaar, I.
    Expression of the nos operon proteins from Pseudomonas stutzeri in transgenic plants to assemble nitrous oxide reductase (2012), Transgenic Res., 21, 593-603.
    View publication on PubMed

Application

Application Comment Organism
agriculture expression of both the senzyme-coding gene nosZ and the mega-cassette of five coding sequences nosFLZDY in Nicotiana tabacum leads to active recombinant N2OR. Extracts from both types of transgenic plants exhibit N2O-reducing activity. The single-gene strategy produces higher reductase capability than the whole-operon approach. Bacterial nitrous oxide reductase expressed in plants could convert N2O into inert N2 without involvement of other Nos proteins Pseudomonas stutzeri

Cloned(Commentary)

Cloned (Comment) Organism
expression in Nicotiana tabacum Pseudomonas stutzeri

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri P19573
-
-
Pseudomonas stutzeri ATCC 14405 P19573
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
453
-
enzyme purified from transgenic Nicotiana tabacum, pH 7.1, 37°C Pseudomonas stutzeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N2O + reduced methyl viologen
-
Pseudomonas stutzeri N2 + oxidized methyl viologen
-
?
N2O + reduced methyl viologen
-
Pseudomonas stutzeri ATCC 14405 N2 + oxidized methyl viologen
-
?

Synonyms

Synonyms Comment Organism
NosZ
-
Pseudomonas stutzeri