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Literature summary for 1.7.2.4 extracted from
- Purification and some characteristics of nitrous oxide reductase from Paracoccus denitrificans (1987), J. Biol. Chem., 262, 6515-6525.
General Stability
| General Stability | Organism |
|---|---|
| turnover dependent activation, nitrite and fluoride accelerate this process | Paracoccus denitrificans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 53% after 30 min at 0.5 mM | Paracoccus denitrificans |  |
| EDTA | 11% at 0.5 mM after 30 min | Paracoccus denitrificans | |
| EGTA | 36% at 0.5 mM after 30 min | Paracoccus denitrificans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
N2O | - |
Paracoccus denitrificans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu | 4.0 atoms per subunit | Paracoccus denitrificans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
2 * 70000, SDS-PAGE | Paracoccus denitrificans |
| 144000 | - |
gel filtration | Paracoccus denitrificans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrous oxide + reduced acceptor | Paracoccus denitrificans | - |
nitrogen + H2O + acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paracoccus denitrificans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification under anaerobic conditions | Paracoccus denitrificans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 122 | - |
anaerobic form | Paracoccus denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrous oxide + reduced acceptor | - |
Paracoccus denitrificans | nitrogen + H2O + acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 70000, SDS-PAGE | Paracoccus denitrificans |
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