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Literature summary for 1.7.2.4 extracted from
- Purification and characterization of a nitrous oxide reductase from Thiosphaera pantotropha. Implications for the mechanism of aerobic nitrous oxide reduction (1993), Eur. J. Biochem., 212, 467-476.
General Stability
| General Stability | Organism |
|---|---|
| an enzyme concentration of at least 0.01 mg per ml is necessary to avoid loss of activity due to dilution | Paracoccus pantotrophus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Acetylene | - |
Paracoccus pantotrophus |  |
| KCN | - |
Paracoccus pantotrophus | |
| NaN3 | - |
Paracoccus pantotrophus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu | wild-type 7.2 atoms per protein dimer | Paracoccus pantotrophus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66281 | - |
2 * 66281, ESI mass spectrometry | Paracoccus pantotrophus |
| 73000 | - |
2 * 73000, SDS-PAGE | Paracoccus pantotrophus |
| 115000 | - |
gel filtration | Paracoccus pantotrophus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrous oxide + reduced acceptor | Paracoccus pantotrophus | - |
nitrogen + H2O + acceptor | - |
? | |
| nitrous oxide + reduced acceptor | Paracoccus pantotrophus LMD 82.5 | - |
nitrogen + H2O + acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paracoccus pantotrophus | - |
LMD 82.5 | - |
| Paracoccus pantotrophus | - |
formerly Thiosphaera pantotropha | - |
| Paracoccus pantotrophus LMD 82.5 | - |
LMD 82.5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of anaerobically grown cells | Paracoccus pantotrophus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1 | - |
ascorbate-reduced phenanzine methosulphate electrode assay | Paracoccus pantotrophus |
| 2.6 | - |
- |
Paracoccus pantotrophus |
| 3.3 | - |
photoreduced methyl viologen spectrophotometric assay | Paracoccus pantotrophus |
| 3.7 | - |
dithionite-reduced methyl viologen electrode assay | Paracoccus pantotrophus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrous oxide + reduced acceptor | - |
Paracoccus pantotrophus | nitrogen + H2O + acceptor | - |
? | |
| nitrous oxide + reduced acceptor | dithionite-reduced methyl viologen as electron donor | Paracoccus pantotrophus | nitrogen + H2O + acceptor | - |
? | |
| nitrous oxide + reduced acceptor | - |
Paracoccus pantotrophus LMD 82.5 | nitrogen + H2O + acceptor | - |
? | |
| nitrous oxide + reduced acceptor | dithionite-reduced methyl viologen as electron donor | Paracoccus pantotrophus LMD 82.5 | nitrogen + H2O + acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 73000, SDS-PAGE | Paracoccus pantotrophus |
| homodimer | 2 * 66281, ESI mass spectrometry | Paracoccus pantotrophus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
above pH 8 with reduced methyl viologen | Paracoccus pantotrophus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000025 | - |
KCN | - |
Paracoccus pantotrophus | |
| 0.00064 | - |
NaN3 | - |
Paracoccus pantotrophus | |
| 0.035 | - |
Acetylene | - |
Paracoccus pantotrophus | |
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