Literature summary for 1.7.2.4 extracted from

Rasmussen, T.; Berks, B.C.; Sanders-Loehr, J.; Dooley, D.M.; Zumft, W.G.; Thomson, A.J.
The catalytic center in nitrous oxide reductase, CuZ, is a copper-sulfide cluster (2000), Biochemistry, 39, 12753-12756.

Search for more literature for 1.7.2.4

Application

Application	Comment	Organism
degradation	plays a critical enviromental role in preventing release into the atmosphere of the potent greenhouse gas nitrous oxide	Pseudomonas stutzeri
degradation	plays a critical enviromental role in preventing release into the atmosphere of the potent greenhouse gas nitrous oxide	Paracoccus pantotrophus

Protein Variants

Protein Variants	Comment	Organism
C622D	no activity, distorted CuA-centre	Pseudomonas stutzeri

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu	wild-type 9.9 atoms per protein dimer	Pseudomonas stutzeri
Cu	wild-type 10.5 atoms per protein dimer	Paracoccus pantotrophus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
nitrous oxide + reduced acceptor	Pseudomonas stutzeri	-	nitrogen + H2O + acceptor	-	?
nitrous oxide + reduced acceptor	Paracoccus pantotrophus	-	nitrogen + H2O + acceptor	-	?

Organism

Organism	UniProt	Comment	Textmining
Paracoccus pantotrophus	-	-	-
Pseudomonas stutzeri	-	wild-type, C622D-mutant laccking copper in the CuA-site and N2OR V a mutant which lacks CuZ	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.5	-	wild-type	Pseudomonas stutzeri
6.9	-	-	Paracoccus pantotrophus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nitrous oxide + reduced acceptor	-	Pseudomonas stutzeri	nitrogen + H2O + acceptor	-	?
nitrous oxide + reduced acceptor	-	Paracoccus pantotrophus	nitrogen + H2O + acceptor	-	?

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Release 2023.1 (January 2023)