Any feedback?
Literature summary for 1.6.2.4 extracted from
- Cloning, functional characterization, and expression profiles of NADPH-cytochrome P450 reductase gene from the Asiatic rice striped stem borer, Chilo suppressalis (Lepidoptera: Pyralidae) (2013), Comp. Biochem. Physiol. B, 166, 225-231.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CsCPR, real-time quantitative PCR expression analysis and phylogenetic tree, heterologous expression of N-terminally truncated and His6-tagged protein in Escherichia coli Rosetta (DE3) cells | Chilo suppressalis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | CsCPR displays Michaelis-Menten behavior with respect to both cytochrome c and NADPH | Chilo suppressalis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound, the N-terminal membrane anchor is functionally dispensable | Chilo suppressalis | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
x * 76000, recombinant N-terminally truncated and His6-tagged protein, SDS-PAGE, x * 77600, about, sequence calculation | Chilo suppressalis |
| 77600 | - |
x * 76000, recombinant N-terminally truncated and His6-tagged protein, SDS-PAGE, x * 77600, about, sequence calculation | Chilo suppressalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADPH + H+ + cytochrome c | Chilo suppressalis | - |
NADP+ + reduced cytochrome c | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chilo suppressalis | R4UB73 | gene CsCPR | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally truncated and His6-tagged protein from Escherichia coli Rosetta (DE3) cells to over 90% purity | Chilo suppressalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood vessel | dorsal | Chilo suppressalis | - |
| fat body | - |
Chilo suppressalis | - |
| head | - |
Chilo suppressalis | - |
| integument | - |
Chilo suppressalis | - |
| leg | - |
Chilo suppressalis | - |
| malpighian tubule | - |
Chilo suppressalis | - |
| midgut | - |
Chilo suppressalis | - |
| additional information | tissue- and developmental stage-dependent expression analysis of CsCPR mRNA by real-time quantitative PCR. The enzyme is exxpressed in the digestive, metabolic, and olfactory organs of the larvae and adults, overview | Chilo suppressalis | - |
| nerve | - |
Chilo suppressalis | - |
| thorax | - |
Chilo suppressalis | - |
| trachea | - |
Chilo suppressalis | - |
| wing | - |
Chilo suppressalis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADPH + H+ + cytochrome c | - |
Chilo suppressalis | NADP+ + reduced cytochrome c | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 76000, recombinant N-terminally truncated and His6-tagged protein, SDS-PAGE, x * 77600, about, sequence calculation | Chilo suppressalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CPR | - |
Chilo suppressalis |
| NADPH-cytochrome P450 reductase | - |
Chilo suppressalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Chilo suppressalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Chilo suppressalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Chilo suppressalis | |
| FMN | - |
Chilo suppressalis | |
| NADPH | - |
Chilo suppressalis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Chilo suppressalis | sequence calculation | - |
5.6 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | NADPH-cytochrome P450 reductase is one of the most important components of the cytochrome P450 enzyme system. It catalyzes electron transfer from NADPH to all known P450s, thus plays central roles not only in the metabolism of exogenous xenobiotics but also in the regulation of endogenous hormones in insects | Chilo suppressalis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
