Cloned (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
deletion mutants | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
E238A/E239A | hinge region connecting the FMN domain to the rest of the protein | Rattus norvegicus |
G237/+AA/E238 | hinge region connecting the FMN domain to the rest of the protein | Rattus norvegicus |
G237/+AAAA/238 | hinge region connecting the FMN domain to the rest of the protein | Rattus norvegicus |
additional information | deletion mutants in the hinge region (connecting the FMN domain to the rest of the protein): deletion of T236 and G237 or deletion of T236, G237, E238 and E239, also in combination with deletion of the first 56 residues of the N-terminus (resulting in a soluble protein) | Rattus norvegicus |
T236A/G237A/E238A/E239A | hinge region connecting the FMN domain to the rest of the protein | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
NADPH | deletion mutant T236/G237/E238/E239 | Rattus norvegicus | |
0.003 | - |
NADPH | deletion mutant T236/G237 | Rattus norvegicus | |
0.0062 | - |
NADPH | - |
Rattus norvegicus | |
0.0135 | - |
cytochrome c | deletion mutant T236/G237 | Rattus norvegicus | |
0.015 | - |
cytochrome c | deletion mutant T236/G237/E238/E239 | Rattus norvegicus | |
0.024 | - |
cytochrome c | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P00388 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
The substitution mutants (E238A/E239A and T236A/G237A/E238A/E239A) exhibit wild-type activity with cytochrome c. The 2- and 4-alanine addition mutants exhibit about 50% increase in activity compared with the wild-type enzyme. The two and four amino acid deletion mutants reduce cytochrome c 6.4- and 214-fold slower than wild type, respectively. | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + H+ + 2 oxidized cytochrome c | - |
Rattus norvegicus | NADP+ + 2 reduced cytochrome c | - |
? | |
NADPH + H+ + 2 oxidized cytochrome P450 2B4 | - |
Rattus norvegicus | NADP+ + 2 reduced cytochrome P450 2B4 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADPH-cytochrome P450 oxidoreductase | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rattus norvegicus | |
FMN | - |
Rattus norvegicus |