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Literature summary for 1.6.2.4 extracted from

  • Hamdane, D.; Xia, C.; Im, S.C.; Zhang, H.; Kim, J.J.; Waskell, L.
    Structure and function of an NADPH-cytochrome P450 oxidoreductase in an open conformation capable of reducing cytochrome P450 (2009), J. Biol. Chem., 284, 11374-11384.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
deletion mutants Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
E238A/E239A hinge region connecting the FMN domain to the rest of the protein Rattus norvegicus
G237/+AA/E238 hinge region connecting the FMN domain to the rest of the protein Rattus norvegicus
G237/+AAAA/238 hinge region connecting the FMN domain to the rest of the protein Rattus norvegicus
additional information deletion mutants in the hinge region (connecting the FMN domain to the rest of the protein): deletion of T236 and G237 or deletion of T236, G237, E238 and E239, also in combination with deletion of the first 56 residues of the N-terminus (resulting in a soluble protein) Rattus norvegicus
T236A/G237A/E238A/E239A hinge region connecting the FMN domain to the rest of the protein Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
NADPH deletion mutant T236/G237/E238/E239 Rattus norvegicus
0.003
-
NADPH deletion mutant T236/G237 Rattus norvegicus
0.0062
-
NADPH
-
Rattus norvegicus
0.0135
-
cytochrome c deletion mutant T236/G237 Rattus norvegicus
0.015
-
cytochrome c deletion mutant T236/G237/E238/E239 Rattus norvegicus
0.024
-
cytochrome c
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P00388
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
The substitution mutants (E238A/E239A and T236A/G237A/E238A/E239A) exhibit wild-type activity with cytochrome c. The 2- and 4-alanine addition mutants exhibit about 50% increase in activity compared with the wild-type enzyme. The two and four amino acid deletion mutants reduce cytochrome c 6.4- and 214-fold slower than wild type, respectively. Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + 2 oxidized cytochrome c
-
Rattus norvegicus NADP+ + 2 reduced cytochrome c
-
?
NADPH + H+ + 2 oxidized cytochrome P450 2B4
-
Rattus norvegicus NADP+ + 2 reduced cytochrome P450 2B4
-
?

Synonyms

Synonyms Comment Organism
NADPH-cytochrome P450 oxidoreductase
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Rattus norvegicus
FMN
-
Rattus norvegicus