Any feedback?
Literature summary for 1.6.2.4 extracted from
- Studies on the microsomal electron-transport system of anaerobically grown yeast. V. Purification and characterization of NADPH-cytochrome c reductase (1977), J. Biochem., 81, 197-205.
General Stability
| General Stability | Organism |
|---|---|
| FAD, FMN necessary for stabilization during purification | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | incubation with NADPH provides protection against inhibition | Saccharomyces cerevisiae |  |
| additional information | - |
Saccharomyces cerevisiae | |
| p-chloromercuribenzoate | incubation with NADPH provides protection against inhibition | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0034 | - |
cytochrome c | - |
Saccharomyces cerevisiae | |
| 0.0324 | - |
NADPH | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Saccharomyces cerevisiae |
| 72000 | - |
1 * 72000, SDS-PAGE | Saccharomyces cerevisiae |
| 82000 | 85000 | calculation from FAD content | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Saccharomyces cerevisiae | microsomal electron transport system composed of cytochrome b5, cytochrome P450, NADH-cytochrome b5 reductase and NADPH-cytochrome c reductase | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
grown anaerobically | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| column chromatography on Sephadex G-150 and DE-52 | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 150 | 180 | - |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Saccharomyces cerevisiae | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c + NADPH | additional electron acceptor: menadione | Saccharomyces cerevisiae | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c + NADPH | additional electron acceptor: 2,6-dichlorophenolindophenol | Saccharomyces cerevisiae | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| ferricytochrome c + NADPH + H+ | - |
Saccharomyces cerevisiae | ferrocytochrome c + NADP+ | - |
r | |
| additional information | microsomal electron transport system composed of cytochrome b5, cytochrome P450, NADH-cytochrome b5 reductase and NADPH-cytochrome c reductase | Saccharomyces cerevisiae | ? | - |
? | |
| NADPH + O2 | slow reaction, presence of menadione, or duroquinone, or vitamin K3 essential | Saccharomyces cerevisiae | NADP+ + O2- | superoxide anion | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 72000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8 | - |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | tightly bound | Saccharomyces cerevisiae | |
| FAD | ratio FAD : FMN 1 : 1 | Saccharomyces cerevisiae | |
| FMN | ratio FAD : FMN 1 : 1 | Saccharomyces cerevisiae | |
| FMN | loosely bound | Saccharomyces cerevisiae | |
| NADPH | - |
Saccharomyces cerevisiae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
