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Literature summary for 1.6.2.4 extracted from

  • Benveniste, I.; Gabriac, B.; Durst, F.
    Purification and characterization of the NADPH-cytochrome P-450 (cytochrome c) reductase from higher-plant microsomal fraction (1986), Biochem. J., 235, 365-373.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.2.4

Activating Compound

Activating Compound Comment Organism Structure
additional information low ionic strength, slight stimulation Helianthus tuberosus

Inhibitors

Inhibitors Comment Organism Structure
additional information high ionic strength Helianthus tuberosus
NADP+ non-competitive inhibition Helianthus tuberosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information cosubstrate NADH, biphasic kinetic Helianthus tuberosus
0.0182
-
 ferricytochrome c determined on the basis of a sequential mechanism Helianthus tuberosus
0.02
-
 NADPH
-
 Helianthus tuberosus
0.0308
-
 NADPH determined on the basis of a sequential mechanism Helianthus tuberosus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Helianthus tuberosus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information stimulation by increasing ionic strength Helianthus tuberosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 differences in MW partially due to method of solubilization Helianthus tuberosus
82000
-
 x * 82000, SDS-PAGE Helianthus tuberosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADPH + ferricytochrome P450 Helianthus tuberosus part of the microsomal electron-transport chains and responsible for the transfer of reducing equivalents between NADPH or NADH and cytochrome P-450 ferrocytochrome P450 + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Helianthus tuberosus
-
 L. var. Blanc commun., Jerusalem artichoke
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange chromatography and affinity chromatography Helianthus tuberosus

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of monooxygenase system Helianthus tuberosus

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Helianthus tuberosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33
-
-
 Helianthus tuberosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Helianthus tuberosus 2 ferrocytochrome c + NADP+ + H+
-
 ?
2 ferricytochrome c + NADPH additional electron acceptor: cytochrome P450 Helianthus tuberosus 2 ferrocytochrome c + NADP+ + H+
-
 ?
ferricytochrome c + NADPH + H+
-
 Helianthus tuberosus ferrocytochrome c + NADP+
-
 r
additional information hydroxylation of coumarate Helianthus tuberosus ?
-
 ?
NADPH + ferricytochrome P450 part of the microsomal electron-transport chains and responsible for the transfer of reducing equivalents between NADPH or NADH and cytochrome P-450 Helianthus tuberosus ferrocytochrome P450 + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 82000, SDS-PAGE Helianthus tuberosus
More differences in subunit weight partially due to method of solubilization Helianthus tuberosus

Cofactor

Cofactor Comment Organism Structure
FAD ratio FAD : FMN 1 : 1 Helianthus tuberosus
FMN ratio FAD : FMN 1 : 1 Helianthus tuberosus
FMN not implicated in plant NADPH-ferricyanide reductase Helianthus tuberosus
NADH low affinity Helianthus tuberosus
NADPH
-
 Helianthus tuberosus