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Literature summary for 1.6.2.4 extracted from

  • Kurokawa, T.; Fukumori, Y.; Yamanaka, T.
    Purification of a flavoprotein having NADPH-cytochrome c reductase and transhydrogenase activities from Nitrobacter winogradskyi and its molecular and enzymatic properties (1987), Arch. Microbiol., 148, 95-99.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
spinach ferredoxin stimulation of the NADPH-reduction of cytochrome c-550 and horse cytochrome c Nitrobacter winogradskyi

Inhibitors

Inhibitors Comment Organism Structure
NAD+ cosubstrate NADH Nitrobacter winogradskyi
NADP+ cosubstrate NADPH Nitrobacter winogradskyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00189
-
NADPH
-
Nitrobacter winogradskyi
1.4
-
4-Nitroacetophenone electron donor NADH, electron acceptor dichlorophenolindophenol Nitrobacter winogradskyi

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Nitrobacter winogradskyi 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
2 * 36000, SDS-PAGE Nitrobacter winogradskyi
70000
-
gel filtration Nitrobacter winogradskyi

Organism

Organism UniProt Comment Textmining
Nitrobacter winogradskyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Nitrobacter winogradskyi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.64
-
electron acceptor: dichlorophenolindophenol Nitrobacter winogradskyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADPH additional electron acceptor: duroquinone Nitrobacter winogradskyi 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: benzylviologen Nitrobacter winogradskyi 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: menadione Nitrobacter winogradskyi 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Nitrobacter winogradskyi 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: duroquinone Nitrobacter winogradskyi ATCC 14123 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: benzylviologen Nitrobacter winogradskyi ATCC 14123 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: menadione Nitrobacter winogradskyi ATCC 14123 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Nitrobacter winogradskyi ATCC 14123 2 ferrocytochrome c + NADP+ + H+
-
?
4-nitroacetophenone + NADPH + H+
-
Nitrobacter winogradskyi ?
-
r
4-nitroacetophenone + NADPH + H+
-
Nitrobacter winogradskyi ATCC 14123 ?
-
r

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Nitrobacter winogradskyi
More N-terminal amino acid sequence is 27% homologous to that of the Spirulina ferredoxin-NADP reductase Nitrobacter winogradskyi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Nitrobacter winogradskyi

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol per subunit Nitrobacter winogradskyi
FMN 0.87 nmol per mg protein Nitrobacter winogradskyi
NADH less effective than NADPH Nitrobacter winogradskyi
NADPH
-
Nitrobacter winogradskyi