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Literature summary for 1.6.2.2 extracted from

  • Marohnic, C.C.; Crowley, L.J.; Davis, C.A.; Smith, E.T.; Barber, M.J.
    Cytochrome b5 reductase: role of the si-face residues, proline 92 and tyrosine 93, in structure and catalysis (2005), Biochemistry, 44, 2449-2461.
    View publication on PubMed
Search for more literature for 1.6.2.2

Protein Variants

Protein Variants Comment Organism
P92A mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
P92G mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
P92S mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
Y93A mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
Y93D mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
Y93F mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
Y93H mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
Y93S mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
Y93W mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0038
-
 NADH mutant Y93S, pH 7.0, 25°C Rattus norvegicus
0.0048
-
 NADH mutant P92A, pH 7.0, 25°C Rattus norvegicus
0.0049
-
 NADH mutant Y93D, pH 7.0, 25°C Rattus norvegicus
0.0058
-
 ferricyanide mutant Y93S, pH 7.0, 25°C Rattus norvegicus
0.006
-
 NADH wild-type, pH 7.0, 25°C Rattus norvegicus
0.0065
-
 NADH mutant P92G, pH 7.0, 25°C Rattus norvegicus
0.0066
-
 ferricyanide mutant Y93F, pH 7.0, 25°C Rattus norvegicus
0.0067
-
 NADH mutant Y93F, pH 7.0, 25°C Rattus norvegicus
0.0068
-
 ferricyanide mutant Y93W, pH 7.0, 25°C Rattus norvegicus
0.0071
-
 ferricyanide wild-type, pH 7.0, 25°C Rattus norvegicus
0.0073
-
 NADH mutant Y93A, pH 7.0, 25°C Rattus norvegicus
0.0074
-
 ferricyanide mutant P92A, pH 7.0, 25°C Rattus norvegicus
0.0077
-
 NADH mutant P92S, pH 7.0, 25°C Rattus norvegicus
0.0078
-
 ferricyanide mutant P92S, pH 7.0, 25°C Rattus norvegicus
0.008
-
 ferricyanide mutant P92G, pH 7.0, 25°C Rattus norvegicus
0.0083
-
 ferricyanide mutant Y93D, pH 7.0, 25°C Rattus norvegicus
0.0083
-
 ferricyanide mutant Y93H, pH 7.0, 25°C Rattus norvegicus
0.0086
-
 ferricyanide mutant Y93A, pH 7.0, 25°C Rattus norvegicus
0.0088
-
 NADH mutant Y93H, pH 7.0, 25°C Rattus norvegicus
0.01
-
 ferricytochrome b5 mutant Y93D, pH 7.0, 25°C Rattus norvegicus
0.01
-
 ferricytochrome b5 mutant Y93H, pH 7.0, 25°C Rattus norvegicus
0.011
-
 ferricytochrome b5 mutant Y93F, pH 7.0, 25°C Rattus norvegicus
0.011
-
 ferricytochrome b5 mutant Y93W, pH 7.0, 25°C Rattus norvegicus
0.012
-
 ferricytochrome b5 mutant P92G, pH 7.0, 25°C Rattus norvegicus
0.012
-
 ferricytochrome b5 mutant P92S, pH 7.0, 25°C Rattus norvegicus
0.012
-
 ferricytochrome b5 mutant Y93S, pH 7.0, 25°C Rattus norvegicus
0.013
-
 ferricytochrome b5 wild-type, pH 7.0, 25°C Rattus norvegicus
0.014
-
 ferricytochrome b5 mutant Y93A, pH 7.0, 25°C Rattus norvegicus
0.015
-
 ferricytochrome b5 mutant P92A, pH 7.0, 25°C Rattus norvegicus
0.054
-
 NADH mutant Y93W, pH 7.0, 25°C Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P20070
-
-

Reaction

Reaction Comment Organism Reaction ID
NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5 Y93 forms contact with the FAD, but neither P92 nor Y93 preceeding the conserved motif RxYTSxxSN coordinating flavin binding are critical for flavin incorporation Rattus norvegicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH
-
 Rattus norvegicus 2 ferrocyanide + NAD+ + H+
-
 ?
2 ferricytochrome b5 + NADH
-
 Rattus norvegicus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14
-
 ferricytochrome b5 mutant Y93D, pH 7.0, 25°C Rattus norvegicus
19
-
 ferricytochrome b5 mutant Y93W, pH 7.0, 25°C Rattus norvegicus
27
-
 ferricytochrome b5 mutant Y93H, pH 7.0, 25°C Rattus norvegicus
32
-
 ferricytochrome b5 mutant Y93S, pH 7.0, 25°C Rattus norvegicus
57
-
 ferricytochrome b5 mutant Y93A, pH 7.0, 25°C Rattus norvegicus
83
-
 ferricyanide mutant Y93D, pH 7.0, 25°C Rattus norvegicus
83
-
 ferricyanide mutant Y93H, pH 7.0, 25°C Rattus norvegicus
83
-
 ferricyanide mutant Y93W, pH 7.0, 25°C Rattus norvegicus
133
-
 ferricyanide mutant Y93S, pH 7.0, 25°C Rattus norvegicus
200
-
 ferricyanide mutant Y93A, pH 7.0, 25°C Rattus norvegicus
295
-
 ferricytochrome b5 mutant Y93F, pH 7.0, 25°C Rattus norvegicus
315
-
 ferricytochrome b5 mutant P92S, pH 7.0, 25°C Rattus norvegicus
320
-
 ferricytochrome b5 mutant P92A, pH 7.0, 25°C Rattus norvegicus
335
-
 ferricytochrome b5 mutant P92G, pH 7.0, 25°C Rattus norvegicus
400
-
 ferricytochrome b5 wild-type, pH 7.0, 25°C Rattus norvegicus
467
-
 ferricyanide mutant P92G, pH 7.0, 25°C Rattus norvegicus
483
-
 ferricyanide mutant Y93F, pH 7.0, 25°C Rattus norvegicus
517
-
 ferricyanide mutant P92A, pH 7.0, 25°C Rattus norvegicus
800
-
 ferricyanide wild-type, pH 7.0, 25°C Rattus norvegicus
880
-
 ferricyanide mutant P92S, pH 7.0, 25°C Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Rattus norvegicus