Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.6.2.2 extracted from

  • Bewley, M.C.; Marohnic, C.C.; Barber, M.J.
    The structure and biochemistry of NADH-dependent cytochrome b5 reductase are now consistent (2001), Biochemistry, 40, 13574-13582.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type, K110R, K110H, K110A, K110E and K110Q mutant enzymes in Escherichia coli Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop method, reservoir: 8% poly ethylene glycol 6000, 5% 2-methyl-2,4-pentanediol in 100 mM 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid, pH 7.5, X-ray structure, resolution: enzyme 2.0 A, enzyme-NAD+ complex, 2.3 A Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
K110A strongly reduced kcat for ferricyanide and cytochrome b5 Rattus norvegicus
K110E strongly reduced kcat for ferricyanide and cytochrome b5 Rattus norvegicus
K110H strongly reduced kcat for ferricyanide and cytochrome b5 Rattus norvegicus
K110Q very low kcat for ferricyanide and cytochrome b5 Rattus norvegicus
K110R reduced kcat for ferricyanid and cytochrome b5 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
cytochrome b5 K110Q mutant enzyme Rattus norvegicus
0.004
-
cytochrome b5 K110E mutant enzyme Rattus norvegicus
0.005
-
ferricyanide K110R mutant enzyme Rattus norvegicus
0.006
-
ferricyanide recombinant wild-type enzyme Rattus norvegicus
0.009
-
cytochrome b5 K110R mutant enzyme Rattus norvegicus
0.009
-
cytochrome b5 K110A mutant enzyme Rattus norvegicus
0.01
-
cytochrome b5 recombinant wild-type enzyme Rattus norvegicus
0.012
-
cytochrome b5 K110H mutant enzyme Rattus norvegicus
0.05
-
ferricyanide K110H mutant enzyme Rattus norvegicus
0.37
-
ferricyanide K110E mutant enzyme Rattus norvegicus
0.38
-
ferricyanide K110A mutant enzyme Rattus norvegicus
0.76
-
ferricyanide K110Q mutant enzyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P20070
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type, K110R, K110H, K110A, K110E and K110Q mutant enzymes Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + ferricytochrome b5
-
Rattus norvegicus NAD+ + H+ + ferrocytochrome b5
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
ferricytochrome b5 K110E mutant enzyme Rattus norvegicus
4
-
ferricytochrome b5 K110Q mutant enzyme Rattus norvegicus
40
-
ferricyanide K110Q mutant enzyme Rattus norvegicus
90
-
ferricytochrome b5 K110A mutant enzyme Rattus norvegicus
110
-
ferricytochrome b5 K110H mutant enzyme Rattus norvegicus
120
-
ferricyanide K110E mutant enzyme Rattus norvegicus
200
-
ferricytochrome b5 K110R mutant enzyme Rattus norvegicus
270
-
ferricytochrome b5 recombinant wild-type enzyme Rattus norvegicus
270
-
ferricyanide K110A mutant enzyme Rattus norvegicus
340
-
ferricyanide K110H mutant enzyme Rattus norvegicus
470
-
ferricyanide K110R mutant enzyme Rattus norvegicus
800
-
ferricyanide recombinant wild-type enzyme Rattus norvegicus