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Literature summary for 1.6.2.2 extracted from
- Detergent-solubilized NADH-cytochrome b5 reductase (1978), Methods Enzymol., 52, 102-108.
General Stability
| General Stability | Organism |
|---|---|
| 0.2% phosphatidycholine liposomes or Triton X-100 stabilize | Oryctolagus cuniculus |
| freezing/thawing: 50% inactivation | Oryctolagus cuniculus |
| Triton X-100 stabilizes | Oryctolagus cuniculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Oryctolagus cuniculus | 5783 | - |
| membrane | enzyme binds in vitro to a variety of membrane preparations, protease treatment removes a hydrophobic segment that is responsible for membrane binding | Oryctolagus cuniculus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE, gel filtration in the presence of 6 M guanidine hydrochloride, gel filtration in the presence of 0.5% sodium deoxycholate | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 622.5 | - |
- |
Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, concentrated solution, several months, no loss of activity | Oryctolagus cuniculus |
| -70°C, several months, no loss of activity | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome b5 + NADH | low activity with trypsin-solubilized cytochrome b5 | Oryctolagus cuniculus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? |  |
| 2 ferricytochrome b5 + NADH | specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol | Oryctolagus cuniculus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| NADH + ferricyanide | - |
Oryctolagus cuniculus | NAD+ + H+ + ferrocyanide | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE, gel filtration in the presence of 6 M guanidine hydrochloride, gel filtration in the presence of 0.5% sodium deoxycholate | Oryctolagus cuniculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 667 | - |
ferricyanide | - |
Oryctolagus cuniculus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Oryctolagus cuniculus | |
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Release 2023.1 (January 2023)
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