KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | 0.0007 | NADH | enzyme from erythrocyte and liver | Homo sapiens | |
0.02 | - |
ferricytochrome b5 | enzyme from eythrocyte membrane | Homo sapiens | |
0.035 | - |
ferricytochrome b5 | enzyme from liver microsome membrane, solubilized with Triton X-100 | Homo sapiens | |
0.04 | - |
ferricytochrome b5 | enzyme from eythrocyte cytosol | Homo sapiens | |
0.045 | - |
ferricytochrome b5 | enzyme from liver microsome membrane, cathepsin D solubilized | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | may be a proteolytic product of the membrane enzyme | Homo sapiens | 5829 | - |
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, enzyme from erythrocyte cytosol and liver microsomal enzyme solubilized with cathepsin D, SDS-PAGE | Homo sapiens |
32000 | - |
x * 32000, enzyme from liver microsomes solubilized with Triton X-100, SDS-PAGE | Homo sapiens |
36000 | - |
x * 36000, enzyme from erythrocyte membrane, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | soluble form | Homo sapiens | - |
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | - |
Homo sapiens | 2 ferrocyanide + NAD+ + H+ | - |
? | |
NADH + ferricytochrome b5 | - |
Homo sapiens | NAD+ + H+ + ferrocytochrome b5 | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, enzyme from erythrocyte cytosol and liver microsomal enzyme solubilized with cathepsin D, SDS-PAGE | Homo sapiens |
? | x * 36000, enzyme from erythrocyte membrane, SDS-PAGE | Homo sapiens |
? | x * 32000, enzyme from liver microsomes solubilized with Triton X-100, SDS-PAGE | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
98.3 | - |
ferricyanide | enzyme from liver microsomes, protease solubilized | Homo sapiens | |
677 | - |
ferricyanide | enzyme from erythrocyte membrane | Homo sapiens | |
700 | - |
ferricyanide | enzyme from erythrocyte cytosol | Homo sapiens | |
827 | - |
ferricyanide | enzyme from liver microsomes, detergent solubilized | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Homo sapiens |